Institute of Molecular Biology, National Chung Cheng University, Chiayi, Taiwan.
J Med Virol. 2011 Dec;83(12):2191-9. doi: 10.1002/jmv.22240.
Human polyomaviruses, JC virus (JCV) and BK virus (BKV), usually remain latent in kidney and urothelial tissue after primary infection. Infection with human polyomavirus has still not been correlated conclusively with malignancy of kidney and urothelial tissue. The present study investigated further the possible relationship between JCV/BKV infection and urothelial carcinoma. Tissue samples were examined from 33 urothelial carcinomas and 5 renal cell carcinomas for JCV/BKV infection, using nested PCR with primers common to both JCV and BKV. The viral genotypes were further verified by endonuclease digestion and DNA sequencing following the PCR. In addition, immunohistochemistry and Western blotting were also performed to detect viral large tumor protein (LT) and the late capsid protein (VP1) in the tissue samples. The results from nested PCR showed that 90.1% (30/33) of the urothelial carcinomas samples and all of the renal cell carcinomas samples (5/5) were JCV DNA positive. Both archetypal and re-arranged JCV genotypes were detected. On the other hand, BKV DNA was detected in only one (3%) of the urothelial carcinoma tissue samples. The immunohistochemical results showed that 30% (10/33) of urothelial carcinoma tissues was stained positive for large tumor antigen (LT). However, the structural protein VP1 was not detectable in any of the tissue samples examined. The present study demonstrated that JCV is highly prevalent in urothelial carcinoma tissue as is the expression of large tumor antigen. Therefore, the findings support the hypothesis that JCV infection is associated with urothelial carcinoma.
人类多瘤病毒，JC 病毒（JCV）和 BK 病毒（BKV），通常在原发感染后在肾脏和尿路上皮组织中保持潜伏。人类多瘤病毒感染与肾和尿路上皮组织的恶性肿瘤之间尚未明确相关。本研究进一步探讨了 JCV/BKV 感染与尿路上皮癌之间的可能关系。使用针对 JCV 和 BKV 共同的引物，通过巢式 PCR 检测 33 例尿路上皮癌和 5 例肾细胞癌组织中 JCV/BKV 感染。通过 PCR 后的内切酶消化和 DNA 测序进一步验证病毒基因型。此外，还进行了免疫组织化学和 Western blot 检测以检测组织样本中的病毒大肿瘤蛋白（LT）和晚期衣壳蛋白（VP1）。巢式 PCR 的结果显示，90.1%（30/33）的尿路上皮癌样本和所有肾细胞癌样本（5/5）均为 JCV DNA 阳性。均检测到原型和重排的 JCV 基因型。另一方面，仅在 1 例（3%）尿路上皮癌组织样本中检测到 BKV DNA。免疫组织化学结果显示，30%（10/33）的尿路上皮癌组织染色阳性，表达大肿瘤抗原（LT）。然而，在检查的任何组织样本中均未检测到结构蛋白 VP1。本研究表明，JCV 在尿路上皮癌组织中高度普遍存在，并且表达大肿瘤抗原。因此，研究结果支持 JCV 感染与尿路上皮癌相关的假说。
