Gu Zhi-Yuan, Li Qi, Si Yi-Ling, Li Xue, Hao Hao-Jie, Song Hai-Jing
Molecular Biology Laboratory, Institute of Basic Medical Sciences, Chinese PLA General Hospital, Beijing, China.
J Med Virol. 2003 Aug;70(4):600-5. doi: 10.1002/jmv.10436.
Several studies have demonstrated that BK virus (BKV) and JC virus (JCV) establish latent infection in peripheral blood leukocytes (PBLs) of healthy individuals; however, the main populations studied are European. In this study, the prevalence of BKV and JCV DNA in PBLs from healthy adult individuals and umbilical cord blood from newborn children in China was detected by semi-nested polymerase chain reaction (snPCR) followed by restriction enzyme analysis. The results suggest that the healthy adult Chinese population harbors BKV and JCV DNA in peripheral leukocytes. Overall, the prevalence of BKV and JCV DNA in PBLs of healthy adult individuals was 42.1% and 7.8%, respectively. The overall prevalence of BKV DNA was significantly higher than that of JCV DNA. None of the umbilical cord blood samples from newborn children were positive for BKV and JCV DNA. To understand further the target tissues involved in establishment of BKV and JCV latency in healthy individuals, the presence of DNA from both viruses was detected in normal arterial wall samples from 20 young trauma victims by the same method used for leukocyte DNA. BKV DNA was detected alone in 20% of samples tested; JCV DNA was not detected alone in any of the samples. DNA from both viruses was found in 5% of samples. This is the first report to show that normal arterial walls of healthy individuals may be another target site of latency for BKV and JCV.
多项研究表明，BK病毒（BKV）和JC病毒（JCV）在健康个体的外周血白细胞（PBL）中建立潜伏感染；然而，主要研究人群是欧洲人。在本研究中，采用半巢式聚合酶链反应（snPCR）及限制性酶切分析检测了中国健康成年个体外周血白细胞和新生儿脐带血中BKV和JCV DNA的流行情况。结果表明，健康的中国成年人群在外周白细胞中携带BKV和JCV DNA。总体而言，健康成年个体外周血白细胞中BKV和JCV DNA的流行率分别为42.1%和7.8%。BKV DNA的总体流行率显著高于JCV DNA。新生儿脐带血样本中均未检测到BKV和JCV DNA阳性。为了进一步了解健康个体中BKV和JCV潜伏感染所涉及的靶组织，采用与检测白细胞DNA相同的方法，在20名年轻创伤受害者的正常动脉壁样本中检测了两种病毒的DNA。在20%的检测样本中单独检测到BKV DNA；在任何样本中均未单独检测到JCV DNA。在5%的样本中发现了两种病毒的DNA。这是首次报道显示健康个体的正常动脉壁可能是BKV和JCV潜伏感染的另一个靶位点。
