Reichl F X, Szinicz L, Kreppel H, Fichtl B, Forth W
Walther Straub-Institut für Pharmakologie and Toxikologie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
Arch Toxicol. 1990;64(4):336-8. doi: 10.1007/BF01972996.
Carbohydrate depletion (glucose and glycogen) was reported to be a major problem in acute arsenic poisoning. In the present paper the effectiveness of glucose substitution was investigated in mice after acute experimental poisoning with As2O3. Four groups of ten mice each received As2O3, 12.9 mg/kg, s.c. After the injection the first group remained without further treatment, the second received saline every 2 h, the third 5% glucose, and the fourth 5% glucose +0.12 IE insulin/kg i.p. Groups 5 and 6, five mice each, received either saline or glucose only. Group 7, five mice, remained without any treatment. Immediately after death the livers were removed for the enzymatic determination of glucose and glycogen. Mice receiving As2O3 only died within 22 h. The mean survival time was 12.4 h. In mice receiving As2O3 and after that saline, glucose, or glucose + insulin, an increase in the survival time to 30.8, 40.7, and 43.6 h, respectively, was observed. All mice which died showed a significant decrease in the liver glucose and glycogen content, compared to control animals. In livers of survivors, the glucose and glycogen content was not different to the control groups. The data support the assumption that carbohydrate depletion is an important factor in arsenic toxicity, and its substitution should be considered in the treatment of arsenic poisoning.
据报道，碳水化合物耗竭（葡萄糖和糖原）是急性砷中毒的一个主要问题。在本文中，研究了在小鼠急性实验性三氧化二砷中毒后葡萄糖替代的有效性。四组，每组十只小鼠，皮下注射12.9mg/kg三氧化二砷。注射后，第一组不做进一步处理，第二组每2小时注射生理盐水，第三组注射5%葡萄糖，第四组腹腔注射5%葡萄糖+0.12 IE胰岛素/kg。第五组和第六组，每组五只小鼠，分别只注射生理盐水或葡萄糖。第七组，五只小鼠，不做任何处理。小鼠死亡后立即取出肝脏，用于酶法测定葡萄糖和糖原。仅接受三氧化二砷的小鼠在22小时内死亡。平均存活时间为12.4小时。在接受三氧化二砷后再注射生理盐水、葡萄糖或葡萄糖+胰岛素的小鼠中，观察到存活时间分别增加到30.8、40.7和43.6小时。与对照动物相比，所有死亡小鼠的肝脏葡萄糖和糖原含量均显著降低。在存活小鼠的肝脏中，葡萄糖和糖原含量与对照组无差异。这些数据支持了碳水化合物耗竭是砷毒性的一个重要因素这一假设，并且在砷中毒治疗中应考虑进行碳水化合物替代。
