Datta S, Mallick P, Bukhsh A R
Kalyani University, Department of Zoology, India.
Complement Ther Med. 1999 Jun;7(2):62-75. doi: 10.1016/s0965-2299(99)80084-3.
To examine the comparative efficacies of Arsenicum Album 30C and 200C and three administrative modes in protecting against the genotoxic effects produced by Arsenic trioxide injection in mice.
Healthy mice, Mus musculus, were intraperitoneally injected with a 0.004% solution of As2O3 @1 ml/100 gms of body weight. Genotoxic effects were assessed through chromosome aberrations (CA), micronucleated erythrocytes (MNE), mitotic index (MI) and sperm head anomaly (SHA) studies, keeping suitable succussed alcohol fed (positive) and As2O3 untreated normal (negative) controls. The As2O3 treated mice were divided into three subgroups, which were orally administered with the drug a) prior to, b) after and c) both prior to and after injection of As2O3 at specific fixation intervals.
While the CA, MNE and SHA were reduced in the drug fed series as compared to respective controls, the MI showed an apparent increase. The combined pre- and post-feeding of Arsenicum album was found to be most effective in reducing the genotoxic effects of As2O3 i200C was more effective than 30C.
Arsenicum Album reduces the genotoxic effect of arsenic poisoning.
研究30C和200C的白砷制剂以及三种给药方式对小鼠注射三氧化二砷所产生的遗传毒性作用的防护效果比较。
选用健康小鼠（小家鼠），腹腔注射0.004%的三氧化二砷溶液，剂量为1毫升/100克体重。通过染色体畸变（CA）、微核红细胞（MNE）、有丝分裂指数（MI）和精子头部异常（SHA）研究评估遗传毒性作用，同时设置合适的振荡酒精喂养（阳性）和未用三氧化二砷处理的正常（阴性）对照。将接受三氧化二砷处理的小鼠分为三个亚组，分别在特定固定时间间隔下，于注射三氧化二砷之前（a组）、之后（b组）以及之前和之后（c组）口服给药。
与各自对照组相比，给药组的CA、MNE和SHA减少，而MI明显增加。发现白砷制剂在注射三氧化二砷之前和之后联合给药对降低三氧化二砷的遗传毒性作用最为有效，200C比30C更有效。
白砷制剂可降低砷中毒的遗传毒性作用。
