Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan Ko Da Pharmaceutical Co., Ltd, Tao-Yuan, Taiwan.
Clin Hemorheol Microcirc. 2011;48(4):211-21. doi: 10.3233/CH-2011-1412.
Free radicals play a critical role in causing hemorheologic abnormality which is highly correlated with cardiovascular disease and stroke. In this study, we established an in vitro model to evaluate the influence of free radical attacks on hemorheological parameters. A well-sealed chamber with hyperbaric oxygen was used to simulate an environment of free radical attacks. Hemorheological parameters, including whole blood viscosity, erythrocyte membrane lipid peroxidation, and erythrocyte deformability, were investigated. We then used the in vitro model to evaluate the anti-free radical effects of some well-known catechin antioxidants, such as epigallocatechin gallate (EGCG), (-)-epicatechin 3-gallate (ECG), and (-)-epigallocatechin (EGC) on abnormal hemorheological parameters induced by hyperbaric oxygen. The results show that an increase in oxygen partial pressure (1.0, 1.5, 2.0 and 2.5 atm) and exposure time (4, 8, 12 and 16 h) resulted in elevated free radical formation and viscosity of whole blood, enhanced lipid peroxidation in erythrocyte membranes, but decreased erythrocyte deformability. In addition, EGCG, ECG, and EGC (0.1, 0.5 and 1.0 μM) effectively ameliorated hemorheologic abnormality and enhanced erythrocyte deformability. Therefore, this study has provided an in vitro hyperbaric oxygen model to rapidly screen or assess the efficacy of functional foods and drugs in the prevention or improvement of hemorheologic abnormality.
自由基在引起血液流变学异常中起着关键作用，而血液流变学异常与心血管疾病和中风高度相关。在这项研究中，我们建立了一个体外模型来评估自由基攻击对血液流变学参数的影响。使用一个密封良好的高压氧室来模拟自由基攻击的环境。研究了血液流变学参数，包括全血粘度、红细胞膜脂质过氧化和红细胞变形性。然后，我们使用该体外模型来评估一些著名儿茶素抗氧化剂，如表没食子儿茶素没食子酸酯（EGCG）、(-)-表儿茶素 3-没食子酸酯（ECG）和(-)-表没食子儿茶素（EGC）对高压氧诱导的异常血液流变学参数的抗自由基作用。结果表明，氧分压（1.0、1.5、2.0 和 2.5 大气压）和暴露时间（4、8、12 和 16 小时）的增加导致自由基形成和全血粘度增加，红细胞膜脂质过氧化增强，但红细胞变形性降低。此外，EGCG、ECG 和 EGC（0.1、0.5 和 1.0 μM）有效改善了血液流变学异常并增强了红细胞变形性。因此，本研究提供了一个体外高压氧模型，可快速筛选或评估功能性食品和药物预防或改善血液流变学异常的功效。
