Azevedo Geraldo Magela, Costa Raquel Alves, Resende Mariana Araujo, Rodrigues Claudiney Melquiades, Vaz Nelson Monteiro, Carvalho Cláudia Rocha
Departamento de Morfologia, ICB-UFMG, Avenue Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, Brazil.
Clin Dev Immunol. 2012;2012:293625. doi: 10.1155/2012/293625. Epub 2011 Oct 13.
Parenteral injection of tolerated proteins into orally tolerant mice inhibits the initiation of immunological responses to unrelated proteins and blocks severe chronic inflammatory reactions of immunological origin, such as autoimmune reactions. This inhibitory effect which we have called "indirect effects of oral tolerance" is also known as "bystander suppression." Herein, we show that i.p. injection of OVA + Al(OH)(3) minutes before i.v. injection of Schistosoma mansoni eggs into OVA tolerant mice blocked the increase of pulmonary granulomas. In addition, the expression of ICAM-1 in lung parenchyma in areas outside the granulomas of OVA-orally tolerant mice was significantly reduced. However, at day 18 after granuloma induction there was no difference in immunofluorescency intensity to CD3, CD4, F4/80, andα-SMA per granuloma area of tolerant and control groups. Reduction of granulomas by reexposure to orally tolerated proteins was not correlated with a shift in Th-1/Th-2 cytokines in serum or lung tissue extract.
向经口服耐受的小鼠进行非口服途径注射耐受蛋白，可抑制对无关蛋白的免疫反应启动，并阻断免疫源性严重慢性炎症反应，如自身免疫反应。我们将这种抑制作用称为“口服耐受的间接效应”，也称作“旁观者抑制”。在此，我们表明，在向经OVA耐受的小鼠静脉注射曼氏血吸虫卵前几分钟腹腔注射OVA + Al(OH)₃，可阻断肺部肉芽肿的增加。此外，经OVA口服耐受小鼠肉芽肿外肺实质中ICAM-1的表达显著降低。然而，在肉芽肿诱导后第18天，耐受组和对照组每个肉芽肿区域对CD3、CD4、F4/80和α-SMA的免疫荧光强度没有差异。再次接触经口服耐受的蛋白导致肉芽肿减少，这与血清或肺组织提取物中Th-1/Th-2细胞因子的变化无关。
