Yan Bei, A Ji-Ye, Hao Hai-Ping, Wang Guang-Ji, Liu Lin-Sheng, Zha Wei-Bin, Zhang Ying, Gu Sheng-Hua
Department of Clinical Pharmacology, Beijing Hospital, Ministry of Health, Beijing 100730, China.
Yao Xue Xue Bao. 2011 Aug;46(8):976-82.
In order to explore the scientific connotation of "Fangzhengduiying (formula corresponding to pattern types)", "Qiyinliangxuzheng (Qi and Yin deficiency pattern)" of myocardial ischemia rat model and GC-TOF/MS based metabonomic method were used for comparing the effects of Sheng-mai injection, Salvia injection and propranolol in the present study. After data processing and pattern recognition, Sheng-mai injection showed better efficacy than the other two drugs in accordance with not only visual observation from PLS-DA scores plots but also the number of abnormal endogenous compounds restored to the normal level. Further studies showed that Sheng-mai injection could normalize the level of plasma endothelin-1, the index related to cardiovascular diseases and sleep disorders, which verified the results of metabonomics. Finally, the regulated metabolites and related metabolic pathways were analyzed, and it was supposed that the effects of Sheng-mai injection involved in the alternation of energy metabolism, lipid metabolism, amino acids metabolism, and so on. These findings provided scientific evidence to Shengmai "Fang" used for "Qi and Yin deficiency pattern" correspondingly, indicating that metabonomics has great potential in traditional Chinese medical research, which provides a novel approach and way to modernization of traditional Chinese medicine.
为探讨“方证对应”的科学内涵，本研究采用基于气相色谱 - 飞行时间质谱联用（GC - TOF/MS）的代谢组学方法，比较生脉注射液、丹参注射液及普萘洛尔对心肌缺血大鼠模型“气阴两虚证”的影响。经过数据处理与模式识别，从偏最小二乘法判别分析（PLS - DA）得分图的直观观察以及恢复至正常水平的内源性异常化合物数量来看，生脉注射液的疗效优于其他两种药物。进一步研究表明，生脉注射液可使与心血管疾病及睡眠障碍相关的指标——血浆内皮素 - 1水平恢复正常，这验证了代谢组学的结果。最后，对调控的代谢产物及相关代谢途径进行分析，推测生脉注射液的作用涉及能量代谢、脂质代谢、氨基酸代谢等的改变。这些研究结果为生脉方对应“气阴两虚证”提供了科学依据，表明代谢组学在中医研究中具有巨大潜力，为中医药现代化提供了新的途径和方法。
