Fang Zhu-yuan, Jiang Wei-min, Zhu Chang-le
Department of Cardiology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Sep;31(9):1201-4.
To study the correlation between Apo E gene polymorphism and patients with coronary heart disease (CHD) of phlegm-stasis syndrome (PSS).
78 CHD patients were assigned to PSS (49 cases) and non-phlegm-stasis syndrome (NPSS). Polymorphisms of Apo E gene in 78 CHD patients and 100 healthy subjects were detected by complete DNA sequencing.
Five gene types as E3/3, E4/4, E2/ 3, E2/4, and E3/4 were detected in the two groups. The frequencies of genotype E3/3 and epsilon 3 allele were significantly lower in CHD patients than in the healthy subjects (P<0.01). But the frequencies of genotype E3/4 and epsilon 4 allele were significantly higher in CHD patients than in the healthy subjects (P<0.01). In CHD patients, the frequencies of genotype E2/4 + E3/4 + E4/4 and epsilon 4 allele were higher in PSS than in NPSS.
Apo E epsilon 4 allele was a susceptible allele to CHD, which was closely correlated to CHD PSS. It was inferred that it might be one of main susceptible alleles for CHD PSS.
研究载脂蛋白E（Apo E）基因多态性与冠心病（CHD）痰瘀证患者的相关性。
将78例冠心病患者分为痰瘀证组（49例）和非痰瘀证组（NPSS）。采用全基因测序法检测78例冠心病患者及100例健康对照者的Apo E基因多态性。
两组均检测出E3/3、E4/4、E2/3、E2/4和E3/4五种基因类型。冠心病患者中E3/3基因型及ε3等位基因频率显著低于健康对照者（P<0.01）；而E3/4基因型及ε4等位基因频率显著高于健康对照者（P<0.01）。在冠心病患者中，痰瘀证组E2/4+E3/4+E4/4基因型及ε4等位基因频率高于非痰瘀证组。
Apo Eε4等位基因是冠心病的易感基因，与冠心病痰瘀证密切相关，推测其可能是冠心病痰瘀证的主要易感基因之一。
