Haematopathology Section, Department of Haematology and Oncology 'L. and A. Seràgnoli', S. Orsola-Malpighi Hospital, University of Bologna, Italy.
Histopathology. 2011 Oct;59(4):679-91. doi: 10.1111/j.1365-2559.2011.03981.x.
To revise 25 cases selected from Karl Lennert's personal archive (21) and Bologna and Frankfurt Registries (four) because of cytological similarities.
All cases were provided with paraffin blocks and studied by immunohistochemistry and molecular techniques. While phenotyping was very informative, among molecular studies only EBER in situ-hybridization (ISH) was successful. Twenty-two cases were concluded as peripheral T cell lymphomas (PTCL). Of these, six were reclassified as angioimmunoblastic T cell lymphoma (AITL), 13 as PTCL, not otherwise specified (NOS), including four follicular variants and one tumour with T-zone pattern, and three as borderline tumours between AITL and PTCL/NOS. All these cases consisted homogeneously of small/medium-sized elements with mild nuclear atypia and an evident rim of clear/pale cytoplasm. On immunohistochemistry, they regularly expressed three to six follicular helper T cell (FTH)-associated markers. EBER-ISH revealed scattered EBV-infected B cells in all tumours except those with 'follicular' growth pattern. The content of follicular dendritic cells and high-endothelial venules varied significantly depending on the histotype.
This study shows that: (i) historical material can be still employed usefully, and (ii) the FTH-phenotype corresponds to a broad spectrum of PTCLs that might form a new category to be validated in future molecular and clinicopathological analyses.
从 Karl Lennert 的个人档案（21 例）和博洛尼亚及法兰克福登记处（4 例）中选择 25 例因细胞学相似而被修订的病例。
所有病例均提供石蜡块，并通过免疫组织化学和分子技术进行研究。虽然表型分析非常有帮助，但在分子研究中，只有 EBER 原位杂交（ISH）成功。22 例被诊断为外周 T 细胞淋巴瘤（PTCL）。其中，6 例被重新分类为血管免疫母细胞性 T 细胞淋巴瘤（AITL），13 例为未特指的 PTCL，包括 4 例滤泡变异型和 1 例具有 T 区模式的肿瘤，3 例为 AITL 和 PTCL/NOS 之间的交界性肿瘤。所有这些病例均由小/中等大小的元素组成，核异型性轻微，具有明显的透明/淡色胞质环。免疫组织化学上，它们通常表达三种至六种滤泡辅助 T 细胞（FTH）相关标志物。除了具有“滤泡”生长模式的肿瘤外，所有肿瘤中均可见散在的 EBV 感染的 B 细胞。滤泡树突状细胞和高内皮静脉的含量根据组织类型而有显著差异。
本研究表明：（i）历史材料仍然可以被有效利用；（ii）FTH 表型对应于广泛的 PTCL，可能形成一个新的类别，需要在未来的分子和临床病理分析中进行验证。
