对植物提取物进行空前超高分辨率羟基 (1)H NMR 光谱分析。

Unprecedented ultra-high-resolution hydroxy group (1)H NMR spectroscopic analysis of plant extracts.

机构信息

Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, GR-45110, Greece.

出版信息

J Nat Prod. 2011 Nov 28;74(11):2462-6. doi: 10.1021/np200329a. Epub 2011 Oct 20.

DOI:10.1021/np200329a

PMID:22014168

Abstract

A general method is demonstrated for obtaining ultra-high resolution in the phenolic hydroxy group 1H NMR spectroscopic region, in DMSO-d6 solution, with the addition of picric acid. Line-width reduction by a factor of over 100 was observed, which resulted in line-widths ranging from 1.6 to 0.6 Hz. This unprecedented resolution, in combination with the shielding sensitivity of the hydroxy group absorptions to substituent effects at least up to 11 bonds distant and the application of 2D 1H-13C HMBC techniques, allows the unequivocal structure analysis of natural products with phenolic hydroxy groups in complex plant extracts.

摘要

本文展示了一种在添加苦味酸的 DMSO-d6 溶液中获得酚羟基 1H NMR 光谱区超高分辨率的通用方法。观察到线宽减小了 100 倍以上，导致线宽从 1.6 到 0.6 Hz 不等。这种前所未有的分辨率，结合羟基吸收对取代基效应的屏蔽敏感性，至少在 11 个键的距离内，以及二维 1H-13C HMBC 技术的应用，使得复杂植物提取物中具有酚羟基的天然产物的结构分析能够得到明确的结果。