铬金属和不锈钢的吸附及蛋白质诱导的金属释放。
Adsorption and protein-induced metal release from chromium metal and stainless steel.
机构信息
Surface and Corrosion Science, Department of Chemistry, Royal Institute of Technology, Drottning Kristinas väg 51, SE-100 44 Stockholm, Sweden.
Surface and Corrosion Science, Department of Chemistry, Royal Institute of Technology, Drottning Kristinas väg 51, SE-100 44 Stockholm, Sweden; Institute for Surface Chemistry, P.O. Box 5607, SE-114 86 Stockholm, Sweden.
出版信息
J Colloid Interface Sci. 2012 Jan 15;366(1):155-164. doi: 10.1016/j.jcis.2011.09.068. Epub 2011 Oct 2.
A research effort is undertaken to understand the mechanism of metal release from, e.g., inhaled metal particles or metal implants in the presence of proteins. The effect of protein adsorption on the metal release process from oxidized chromium metal surfaces and stainless steel surfaces was therefore examined by quartz crystal microbalance with energy dissipation monitoring (QCM-D) and graphite furnace atomic absorption spectroscopy (GFAAS). Differently charged and sized proteins, relevant for the inhalation and dermal exposure route were chosen including human and bovine serum albumin (HSA, BSA), mucin (BSM), and lysozyme (LYS). The results show that all proteins have high affinities for chromium and stainless steel (AISI 316) when deposited from solutions at pH 4 and at pH 7.4 where the protein adsorbed amount was very similar. Adsorption of albumin and mucin was substantially higher at pH 4 compared to pH 7.4 with approximately monolayer coverage at pH 7.4, whereas lysozyme adsorbed in multilayers at both investigated pH. The protein-surface interaction was strong since proteins were irreversibly adsorbed with respect to rinsing. Due to the passive nature of chromium and stainless steel (AISI 316) surfaces, very low metal release concentrations from the QCM metal surfaces in the presence of proteins were obtained on the time scale of the adsorption experiment. Therefore, metal release studies from massive metal sheets in contact with protein solutions were carried out in parallel. The presence of proteins increased the extent of metals released for chromium metal and stainless steel grades of different microstructure and alloy content, all with passive chromium(III)-rich surface oxides, such as QCM (AISI 316), ferritic (AISI 430), austentic (AISI 304, 316L), and duplex (LDX 2205).
人们开展了一项研究工作，旨在了解在蛋白质存在的情况下，例如吸入的金属颗粒或金属植入物，金属释放的机制。因此，通过石英晶体微天平（QCM）与能量耗散监测（QCM-D）和石墨炉原子吸收光谱（GFAAS）研究了蛋白质吸附对氧化铬金属表面和不锈钢表面金属释放过程的影响。选择了具有不同电荷和大小的蛋白质，这些蛋白质与吸入和皮肤暴露途径有关，包括人血清白蛋白（HSA）、牛血清白蛋白（BSA）、粘蛋白（BSM）和溶菌酶（LYS）。结果表明，所有蛋白质在 pH 4 和 pH 7.4 从溶液中沉积时对铬和不锈钢（AISI 316）都具有很高的亲和力。与 pH 7.4 相比，白蛋白和粘蛋白在 pH 4 时的吸附量要高得多，在 pH 7.4 时吸附量约为单层，而溶菌酶在两个研究 pH 值下均以多层形式吸附。由于蛋白质相对于冲洗是不可逆吸附的，因此蛋白质与表面的相互作用很强。由于铬和不锈钢（AISI 316）表面的被动性质，在吸附实验的时间尺度内，从 QCM 金属表面在蛋白质存在下获得的金属释放浓度非常低。因此，在与蛋白质溶液接触的大量金属板上进行了金属释放研究。蛋白质的存在增加了不同微观结构和合金含量的铬金属和不锈钢等级释放的金属量，所有这些都具有富铬（III）的被动表面氧化物，例如 QCM（AISI 316）、铁素体（AISI 430）、奥氏体（AISI 304、316L）和双相（LDX 2205）。
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