Pharmacy School of Shanghai Jiao Tong University, Shanghai, China.
Bioorg Med Chem Lett. 2011 Dec 1;21(23):7175-9. doi: 10.1016/j.bmcl.2011.09.070. Epub 2011 Sep 29.
We report the development of a synthetic, biotin-conjugated diadenosine tetraphosphate (Ap(4)A)-'molecular hook' attached to magnetic beads enabling the isolation of Ap(4)A-binding proteins from bacterial cells or mammalian tissue lysates. Characterisation and identification of isolated binding proteins is performed sequentially by mass spectrometry. The observation of positive controls suggests that these newly observed proteins are putative Ap(4)A-binding partners, and we have expectations that others can be found with further technical improvements in our methods.
我们报告了一种合成的、生物素缀合的二腺苷四磷酸（Ap(4)A）-“分子钩”的开发，该“分子钩”连接到磁珠上，可从细菌细胞或哺乳动物组织裂解物中分离 Ap(4)A 结合蛋白。通过质谱法依次对分离的结合蛋白进行特征描述和鉴定。阳性对照的观察表明，这些新观察到的蛋白质是潜在的 Ap(4)A 结合伴侣，并且我们期望在我们的方法进一步技术改进后，可以发现其他的 Ap(4)A 结合伴侣。
