Department of Pharmacognosy and Environmental Medicine, University of Nigeria, Nsukka, Nigeria.
Asian Pac J Trop Med. 2011 Oct;4(10):791-5. doi: 10.1016/S1995-7645(11)60195-8.
To evaluate the anti-inflammatory property of leaves of Sansevieria liberica Ger. and Labr. and to ascertain the toxicity and phytochemical profiles of the extract of the leaves.
The juice from the fresh leaves was expressed manually and lyophilized. The crude extract (CE) was then fractionated into n-hexane fraction (HF), chloroform fraction (CF), ethylacetate fraction (EF) and methanol fraction (MF). The crude extract (CF) and the fractions were screened for anti-inflammatory activity using egg albumen-induced paw (systemic) edema in rats as a measure of acute inflammation. The toxicity test and phytochemical screening were done using standard procedures.
The CE and the fractions significantly (P<0.05) inhibited the development of paw edema induced by egg albumen in rats. The potency/activity of the CE and the fractions increased in the order HF>CE>MF>CF>EF, with the CE and HF at 400 mg/kg exhibiting inhibition comparable to that obtained with 5 mg/kg diclofenac sodium. Acute toxicity test on CE established an oral and intraperitoneal LD(50) of > 5 000 mg/kg in mice. Phytochemical screening of the CE and the fractions showed the presence of various bioactive substances such as alkaloids, saponins, flavonoids, terpenoids, steroids, glycosides, reducing sugars, tannins, resins, carbohydrates, proteins, acidic compounds, fats and oils.
The results of the study showed that the leaves of Sansevieria liberica Ger and Labr. possess anti-inflammatory effects which may be due to its bioactive constituents. Further purification on these bioactive constituents may result in the development of potent anti-inflammatory agent with low toxicity and better therapeutic index.
评估虎尾兰的叶片的抗炎特性，并确定叶片提取物的毒性和植物化学成分。
手动压榨新鲜叶片并冻干得到叶片汁，然后将粗提取物（CE）进一步分离为正己烷部分（HF）、氯仿部分（CF）、乙酸乙酯部分（EF）和甲醇部分（MF）。使用蛋清诱导的大鼠爪（全身）水肿作为急性炎症的衡量标准，对粗提取物（CF）和各部分进行抗炎活性筛选。使用标准程序进行毒性测试和植物化学成分筛选。
CE 和各部分显著（P<0.05）抑制了蛋清诱导的大鼠爪水肿的发展。CE 和各部分的效力/活性顺序为 HF>CE>MF>CF>EF，CE 和 HF 在 400mg/kg 时的抑制作用与 5mg/kg 双氯芬酸钠相当。CE 的急性毒性试验在小鼠中确定口服和腹腔内 LD(50)大于 5000mg/kg。CE 和各部分的植物化学成分筛选显示存在各种生物活性物质，如生物碱、皂苷、类黄酮、萜类、甾体、糖苷、还原糖、单宁、树脂、碳水化合物、蛋白质、酸性化合物、脂肪和油。
研究结果表明，虎尾兰的叶片具有抗炎作用，这可能与其生物活性成分有关。对这些生物活性成分进行进一步纯化可能会开发出具有低毒性和更好治疗指数的有效抗炎剂。
