Department of Pharmacology, Sinhgad College of Pharmacy, Pune, India.
J Ethnopharmacol. 2012 Aug 1;142(3):627-33. doi: 10.1016/j.jep.2012.05.007. Epub 2012 Jun 7.
The leaves and rhizomes of Sansevieria trifasciata are used in folk medicine for treating bronchitis, asthma, cough, snake bite and insect bite etc. The ethanolic extract elicited analgesic, anti-inflammatory and antipyretic activity. Hence, it was decided to study the antiallergic activity of ethanolic extract of S. trifasciata (EEST) on various animal models as well as in vitro conditions, and also to understand possible mechanism of action.
Ethanolic extract of S. trifasciata leaves (EEST) were prepared by cold maceration followed by concentration and evaporation under reduced pressure on a rotary evaporator to obtain semisolid mass. The various phytoconstituents were analyzed. The acute toxicity study of EEST was carried out in mice. The antiallergic and anaphylactic activities were evaluated using animal models viz. milk induced eosinophilia and leukocytosis, compound 48/80 induced mast cell degranulation, active and passive cutaneous anaphylaxis and histamine induced pedal edema. In addition, EEST effect on Shultz-Dale reaction in sensitized guinea pig ileum in ex vivo and antioxidant activity by free radical scavenging by DPPH method (in vitro) were also studied.
EEST treatment at 100mg/kg and 200mg/kg p.o inhibited (a) milk-induced increased eosinophilia, leukocytosis, monocytes and neutrophils. (b) Prevented passive cutaneous and active anaphylactoid reactions. (c) Prevented compound 48/80 induced degranulation of sensitized mesenteric mast cells. (d) Inhibited histamine induced pedal edema formation significantly. EEST pretreatment inhibited Shultz-Dale reaction in guinea pig ileum and also elicited potent antioxidant activity.
Experimental findings demonstrate promising antiallergic and anti-anaphylactic activity of EEST and also elicited potent antioxidant activity. The antiallergic and anti-anphylactic activity might be due to inhibition of release of chemical mediators from mast cells largely by phytoconstituents like steroidal saponins, triterpenoids and flavonoids present in EEST.
龙舌兰三裂叶的叶子和根茎在民间医学中用于治疗支气管炎、哮喘、咳嗽、蛇咬伤和昆虫咬伤等。乙醇提取物具有镇痛、抗炎和退热作用。因此，决定研究龙舌兰三裂叶乙醇提取物（EEST）在各种动物模型以及体外条件下的抗过敏活性，并了解可能的作用机制。
通过冷浸和减压旋转蒸发浓缩蒸发，从龙舌兰三裂叶中提取乙醇提取物（EEST），得到半固体物质。分析各种植物成分。进行 EEST 的急性毒性研究。使用动物模型评估抗过敏和过敏反应活性，即牛奶诱导的嗜酸性粒细胞增多和白细胞增多、化合物 48/80 诱导肥大细胞脱颗粒、主动和被动皮肤过敏反应以及组胺诱导的足垫水肿。此外，还研究了 EEST 对致敏豚鼠回肠离体 Shultz-Dale 反应的影响以及通过 DPPH 法（体外）清除自由基的抗氧化活性。
EEST 以 100mg/kg 和 200mg/kg 口服治疗可抑制：（a）牛奶诱导的嗜酸性粒细胞增多、白细胞增多、单核细胞和中性粒细胞增多。（b）预防被动皮肤和主动过敏样反应。（c）预防化合物 48/80 诱导的致敏肠系膜肥大细胞脱颗粒。（d）显著抑制组胺诱导的足垫水肿形成。EEST 预处理抑制了豚鼠回肠的舒尔茨-戴尔反应，并表现出强大的抗氧化活性。
实验结果表明 EEST 具有有前景的抗过敏和抗过敏反应活性，并表现出强大的抗氧化活性。抗过敏和抗过敏反应活性可能是由于植物成分如甾醇皂苷、三萜类化合物和类黄酮抑制肥大细胞释放化学介质所致。
