Parasitology Department, Institute of Medicine Tropical Pedro Kourí, Havana City, Cuba.
Asian Pac J Trop Med. 2011 Oct;4(10):836-40. doi: 10.1016/S1995-7645(11)60204-6.
To evaluate the antileishmanial activity of different extracts from three Cuban Pluchea species.
In in vitro assays the IC(50) was calculated in the promastigotes and amastigotes forms as cytotoxicity in murine macrophages. In leishmaniasis cutanea experiment, mortality, weight loss, lesion size and burden parasite were measured.
Extracts evaluated showed inhibitive effect on growing of promastigote form; however, active extracts caused a high toxicity. Ethanol and n-hexane extracts demonstrated specific antileishmanial activity. Ethanol and n-hexane extracts from Pluchea carolinensis (P. carolinensis) caused similar inhibition against amastigote form. The intraperitoneal administration of the ethanol extract of P. carolinensis at 100 mg/kg prevented lesion development compared with control groups.
The antileishmanial experiment suggests that ethanol extracts from P. carolinensis is the most promising. Further studies are still needed to evaluate the potential of this plant as a source of new antileishmanial agents.
评估三种古巴蓬属植物不同提取物的抗利什曼原虫活性。
在体外试验中，通过计算在原虫和无鞭毛体形式的细胞毒性来计算 IC（50）值。在皮肤利什曼病实验中，测量死亡率、体重减轻、病变大小和寄生虫负荷。
评估的提取物对前鞭毛体的生长表现出抑制作用；然而，活性提取物具有高毒性。乙醇和正己烷提取物表现出特异性抗利什曼原虫活性。来自蓬属（蓬属）的乙醇和正己烷提取物对无鞭毛体形式有相似的抑制作用。与对照组相比，100mg/kg 剂量的蓬属乙醇提取物腹腔给药可预防病变发展。
抗利什曼原虫实验表明，蓬属的乙醇提取物最有希望。仍需要进一步研究来评估这种植物作为新型抗利什曼原虫药物来源的潜力。
