Pekovits Karin, Wildburger Angelika, Payer Michael, Hutter Heinz, Jakse Norbert, Dohr Gottfried
Institute of Cell Biology, Histology, and Embryology, Medical University of Graz, Graz, Austria.
J Oral Maxillofac Surg. 2012 Jan;70(1):154-62. doi: 10.1016/j.joms.2011.07.019. Epub 2011 Oct 19.
The aim of the present study was to compare the influence of 2 different bone scrapers with respect to graft quality.
The study was conducted as a prospective, controlled experimental study of patients selected from the outpatient unit of the Department of Oral Surgery and Radiology (Dental Clinic, Medical University, Graz, Austria). Bone samples were obtained during routine lower third molar removal. Both a manual bone scraper (MS) and a piezoelectric device (PD) were used in directly adjacent regions in each case. As variables, the chip morphology, cell viability, and osteogenic differentiation were investigated. For statistical analysis, the Student t test and Fisher's exact test (P < .05) were applied.
A total of 20 patients (12 women and 8 men, mean age 28.15 ± 5.8 years) were included in the study. A series of 40 bone samples was obtained during lower third molar removal. MS and PD enabled similar intraoral harvest of bone chips. In vitro outgrowth of adherent cells was found in 90% of the MS and 80% of the PD samples after 7 to 18 days, without statistical significance (P = .67). Similar cell viability of outgrowing cells in both groups was observed (94.7% ± 2.2% in the MS group and 94.1% ± 1.6% in the PD group). Reverse transcriptase-polymerase chain reaction analysis and the staining pattern verified osteopotent cells in both groups.
Both manual and piezoelectric techniques are adequate harvesting technologies for limited intraoral augmentations. Our results did not show an advantage for the piezoelectric device.
本研究旨在比较两种不同骨刮匙对移植骨质量的影响。
本研究为前瞻性对照实验研究，研究对象选自奥地利格拉茨医科大学牙科诊所口腔外科和放射科门诊患者。在常规拔除下颌第三磨牙过程中获取骨样本。在每种情况下，均在紧邻区域分别使用手动骨刮匙（MS）和压电装置（PD）。将骨片形态、细胞活力和成骨分化作为变量进行研究。采用Student t检验和Fisher精确检验进行统计学分析（P <.05）。
本研究共纳入20例患者（12例女性，8例男性，平均年龄28.15±5.8岁）。在拔除下颌第三磨牙过程中获取了一系列40块骨样本。MS和PD获取的口腔内骨片量相似。7至18天后，90%的MS样本和80%的PD样本出现贴壁细胞的体外生长，差异无统计学意义（P =.67）。两组中生长细胞的细胞活力相似（MS组为94.7%±2.2%，PD组为94.1%±1.6%）。逆转录聚合酶链反应分析和染色模式证实两组中均存在骨祖细胞。
对于有限的口腔内植骨，手动技术和压电技术均为合适的采集技术。我们的结果未显示压电装置具有优势。
