Zaffe Davide, D'Avenia Ferdinando
Department of Anatomy and Histology, Section of Human Anatomy, University of Modena and Reggio Emilia, Modena, Italy.
Clin Oral Implants Res. 2007 Aug;18(4):525-33. doi: 10.1111/j.1600-0501.2007.01368.x. Epub 2007 Apr 18.
To evaluate histologically the morphology and characteristics of bone chips harvested intraorally by Safescraper, a specially designed cortical bone collector.
Bone chips harvested near a bone defect or in other intraoral sites were grafted into a post-extractive socket or applied in procedures for maxillary sinus floor augmentation or guided bone regeneration. Core biopsies were performed at implant insertion. Undecalcified specimens embedded in PMMA were studied by histology, histochemistry and SEM.
Intraoral harvesting by Safescraper provided a simple, clinically effective regenerative procedure with low morbidity for collecting cortical bone chips (0.9-1.7 mm in length, roughly 100 microm thick). Chips had an oblong or quadrangular shape and contained live osteocytes (mean viability: 45-72%). Bone chip grafting produced newly formed bone tissue suitable for implant insertion. Trabecular bone volume measured on biopsies decreased with time (from 45-55% to 23%). Grafted chips made up 50% or less of the calcified tissue in biopsies. Biopsies presented remodeling activities, new bone formation by apposition and live osteocytes (35% or higher).
In conclusion, Safescraper is capable of collecting adequate amounts of cortical bone chips from different intraoral sites. The procedure is effective for treating alveolar defects for endosseous implant insertion and provides good healing of small bone defects after grafting with bone chips. The study indicates that Safescraper is a very useful device for in-office bone harvesting procedures in routine peri-implant bone regeneration.
通过组织学评估一种专门设计的皮质骨采集器Safescraper在口腔内采集的骨屑的形态和特征。
将在骨缺损附近或其他口腔内部位采集的骨屑移植到拔牙后的牙槽窝中，或应用于上颌窦底提升或引导骨再生手术。在植入种植体时进行核心活检。对嵌入聚甲基丙烯酸甲酯（PMMA）的未脱钙标本进行组织学、组织化学和扫描电子显微镜（SEM）研究。
使用Safescraper在口腔内采集骨屑提供了一种简单、临床有效的再生方法，采集皮质骨屑的发病率低（长度为0.9 - 1.7毫米，厚度约为100微米）。骨屑呈长方形或四边形，含有活的骨细胞（平均活力：45 - 72%）。骨屑移植产生了适合植入种植体的新形成的骨组织。活检时测量的小梁骨体积随时间减少（从45 - 55%降至23%）。移植的骨屑在活检的钙化组织中占比50%或更少。活检显示有重塑活动、通过贴附形成新骨以及活的骨细胞（35%或更高）。
总之，Safescraper能够从不同的口腔内部位采集足够数量的皮质骨屑。该方法对于治疗用于骨内种植体植入的牙槽骨缺损有效，并且在用骨屑移植后能使小骨缺损良好愈合。该研究表明，Safescraper是常规种植体周围骨再生门诊骨采集手术中非常有用的设备。
