University Clinic of Respiratory and Allergic Diseases Colnik, Colnik, Slovenia.
Respir Med. 2011 Oct;105 Suppl 1:S81-3. doi: 10.1016/S0954-6111(11)70017-8.
Exposure to acetylsalicylic acid (ASA) may exacerbate respiratory or skin diseases or induce anaphylactoid reactions in apparently healthy individuals. We wanted to evaluate the clinical and diagnostic utility of measuring ASA-induced 15-hydroxyeicosatetraenoic acid (15-HETE) generation.
We performed a prospective single-blind study with 26 subjects undergoing clinical evaluation and/or ASA provocation testing. We also included 12 control subjects. Peripheral blood leukocytes were incubated with 500 μM ASA and 15-HETE release was measured by competitive ELISA.
We found that 18 subjects were ASA-tolerant and 8 were ASA-intolerant. The mean increase in 15-HETE in intolerant subjects was 34% and this was comparable to the mean increase of 30% observed in ASA-tolerant subjects. A similar mean increase was also observed in control subjects. The ROC calculation showed that the optimal diagnostic threshold would be an increase of greater than 33%. However, the sensitivity of this increase was only 63% and the specificity was 50%.
Our data suggest that further studies are needed before the ASA-induced 15-HETE test can be used in clinical practice.
在看似健康的个体中，接触乙酰水杨酸（ASA）可能会加重呼吸道或皮肤疾病或引发过敏样反应。我们旨在评估测量 ASA 诱导的 15-羟二十碳四烯酸（15-HETE）生成的临床和诊断效用。
我们进行了一项前瞻性单盲研究，共纳入 26 名接受临床评估和/或 ASA 激发试验的受试者，还纳入了 12 名对照受试者。外周血白细胞用 500μM ASA 孵育，并通过竞争性 ELISA 测量 15-HETE 的释放。
我们发现 18 名受试者对 ASA 耐受，8 名受试者对 ASA 不耐受。不耐受受试者的 15-HETE 平均增加 34%，与 ASA 耐受受试者观察到的平均增加 30%相当。对照受试者也观察到类似的平均增加。ROC 计算表明，最佳诊断阈值为增加大于 33%。然而，这种增加的敏感性仅为 63%，特异性为 50%。
我们的数据表明，在 ASA 诱导的 15-HETE 试验可用于临床实践之前，还需要进一步研究。
