Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8392, USA.
Anal Bioanal Chem. 2012 Jan;402(2):749-62. doi: 10.1007/s00216-011-5447-8. Epub 2011 Oct 21.
Standard Reference Material 968e Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum provides certified values for total retinol, γ- and α-tocopherol, total lutein, total zeaxanthin, total β-cryptoxanthin, total β-carotene, 25-hydroxyvitamin D(3), and cholesterol. Reference and information values are also reported for nine additional compounds including total α-cryptoxanthin, trans- and total lycopene, total α-carotene, trans-β-carotene, and coenzyme Q(10). The certified values for the fat-soluble vitamins and carotenoids in SRM 968e were based on the agreement of results from the means of two liquid chromatographic methods used at the National Institute of Standards and Technology (NIST) and from the median of results of an interlaboratory comparison exercise among institutions that participate in the NIST Micronutrients Measurement Quality Assurance Program. The assigned values for cholesterol and 25-hydroxyvitamin D(3) in the SRM are the means of results obtained using the NIST reference method based upon gas chromatography-isotope dilution mass spectrometry and liquid chromatography-isotope dilution tandem mass spectrometry, respectively. SRM 968e is currently one of two available health-related NIST reference materials with concentration values assigned for selected fat-soluble vitamins, carotenoids, and cholesterol in human serum matrix. This SRM is used extensively by laboratories worldwide primarily to validate methods for determining these analytes in human serum and plasma and for assigning values to in-house control materials. The value assignment of the analytes in this SRM will help support measurement accuracy and traceability for laboratories performing health-related measurements in the clinical and nutritional communities.
标准参考物质 968e 人血清中脂溶性维生素、类胡萝卜素和胆固醇提供了总视黄醇、γ-和α-生育酚、叶黄素总量、玉米黄质总量、β-隐黄质总量、β-胡萝卜素总量、25-羟维生素 D(3)和胆固醇的认证值。还报告了参考和信息值，用于另外 9 种化合物，包括总α-隐黄质、反式和全番茄红素、总α-胡萝卜素、反式β-胡萝卜素和辅酶 Q(10)。SRM 968e 中脂溶性维生素和类胡萝卜素的认证值基于两种液相色谱方法的平均值，这两种方法都由美国国家标准与技术研究院（NIST）使用，并且还基于参与 NIST 微量营养素测量质量保证计划的机构之间的实验室间比较活动的结果的中位数。SRM 中胆固醇和 25-羟维生素 D(3)的赋值值是使用基于气相色谱-同位素稀释质谱法和液相色谱-同位素稀释串联质谱法的 NIST 参考方法获得的结果的平均值。SRM 968e 是目前两种可用于健康相关的 NIST 参考物质之一，其在人血清基质中具有选定的脂溶性维生素、类胡萝卜素和胆固醇的浓度值。该标准物质被世界各地的实验室广泛使用，主要用于验证人血清和血浆中这些分析物的测定方法，并为内部对照材料赋值。该标准物质中分析物的赋值将有助于支持在临床和营养界进行健康相关测量的实验室的测量准确性和可追溯性。
