Thomas Jeanice B, Sharpless Katherine E, Yen James H, Rimmer Catherine A
National Institute of Standards and Technology, Analytical Chemistry Division, Gaithersburg, MD 20899-8392, USA.
J AOAC Int. 2011 May-Jun;94(3):815-22.
The concentrations of selected fat-soluble vitamins and carotenoids in Standard Reference Material (SRM) 3280 Multivitamin/Multielement Tablets have been determined by two independent LC methods, with measurements performed by the National Institute of Standards and Technology (NIST). This SRM has been prepared as part of a collaborative effort between NIST and the National Institutes of Health Office of Dietary Supplements. The SRM is also intended to support the Dietary Supplement Ingredient Database that is being established by the U.S. Department of Agriculture. The methods used at NIST to determine the concentration levels of vitamins A and E, and beta-carotene in the SRM used RPLC with absorbance detection. The relative precision of these methods ranged from 2 to 8% for the analytes measured. SRM 3280 is primarily intended for use in validating analytical methods for the determination of selected vitamins, carotenoids, and elements in multivitamin/multielement tablets and similar matrixes.
美国国家标准与技术研究院（NIST）采用两种独立的液相色谱法测定了标准参考物质（SRM）3280多种维生素/多种元素片剂中选定的脂溶性维生素和类胡萝卜素的浓度。该标准参考物质是NIST与美国国立卫生研究院膳食补充剂办公室合作制备的一部分。该标准参考物质还旨在支持美国农业部正在建立的膳食补充剂成分数据库。NIST用于测定该标准参考物质中维生素A、维生素E和β-胡萝卜素浓度水平的方法采用了带吸光度检测的反相液相色谱法。所测分析物的这些方法的相对精密度在2%至8%之间。SRM 3280主要用于验证测定多种维生素/多种元素片剂及类似基质中选定维生素、类胡萝卜素和元素的分析方法。
