Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
Cell Stress Chaperones. 2012 Jan;17(1):131-8. doi: 10.1007/s12192-011-0294-2. Epub 2011 Oct 21.
Abnormal levels of heat shock proteins have been observed in a number of human neoplasms and demonstrate prognostic, predictive and therapeutic implications. Since osteosarcoma (OSA) in dogs provides an important model for the same disease in humans, the aim of this study was to evaluate the immunohistochemical expression of Hsp27, Hsp72, Hsp73 and Hsp90 in 18 samples of canine appendicular OSA, in relation to histological grade and overall survival (OS), in order to investigate their potential prognostic, predictive and/or therapeutic value. A semiquantitative method was used for the analysis of the results. Hsp27, Hsp73 and Hsp90 showed a variably intense, cytoplasmic and nuclear immunoreactivity that was not associated with histological type or grade. On the other hand, a high percentage of Hsp72 immunostaining was significantly associated with grade III (P < 0.01) and a lack of immunolabelling was significantly correlated to a longer OS (P = 0.006). Neoplastic emboli were occasionally positive for Hsp27, faintly immunoreactive for Hsp72 and intensely immunolabelled by Hsp73 and Hsp90. In conclusion, absence of Hsp72 immunosignal appears to be associated with a favourable prognosis whilst the widespread Hsp90 immunoreactivity detected in all tumour cases as well as in neoplastic emboli, suggests this protein could be targeted in the therapy of canine OSA, and likewise in its human counterpart.
已在多种人类肿瘤中观察到热休克蛋白水平异常，这些异常具有预后、预测和治疗意义。由于犬骨肉瘤（OSA）为人类同种疾病提供了重要模型，因此本研究旨在评估 18 例犬四肢 OSA 组织中 Hsp27、Hsp72、Hsp73 和 Hsp90 的免疫组化表达与组织学分级和总生存期（OS）的关系，以研究它们在预后、预测和/或治疗方面的潜在价值。采用半定量方法分析结果。Hsp27、Hsp73 和 Hsp90 显示出不同强度的细胞质和核内免疫反应性，但与组织学类型或分级无关。另一方面，高百分比的 Hsp72 免疫染色与 3 级显著相关（P<0.01），缺乏免疫标记与更长的 OS 显著相关（P=0.006）。肿瘤栓子偶尔对 Hsp27 呈阳性，对 Hsp72 呈弱阳性，对 Hsp73 和 Hsp90 呈强阳性。总之，缺乏 Hsp72 免疫信号似乎与预后良好相关，而在所有肿瘤病例以及肿瘤栓子中检测到的广泛的 Hsp90 免疫反应性表明，该蛋白可作为犬 OSA 治疗的靶点，同样也可作为其人类对应物的治疗靶点。
