TNF-α -308G/A 多态性与墨西哥人群 2 型糖尿病家族史的关联。
Association of the TNF-α -308G/A polymorphism with family history of type 2 diabetes mellitus in a Mexican population.
机构信息
Departamento de Ciencias Medicas, Universidad de Guanajuato, Mexico.
出版信息
Clin Biochem. 2012 Jan;45(1-2):12-5. doi: 10.1016/j.clinbiochem.2011.09.018. Epub 2011 Oct 8.
AIMS
We examined the possible association of the -308G/A polymorphism of the TNF-α promoter gene in type 2 diabetes mellitus (DM2) patients and in non-diabetic subjects with and without family history of DM2.
METHODS
We studied 87 non-diabetic subjects without DM2 family history in at least one of two generations, 48 non-diabetic subjects with DM2 family history and 95 DM2 patients. Genotyping was carried out by PCR-RFLP.
RESULTS
The frequency of TNF-α -308G/A genotype was significantly lower in non-diabetic subjects without DM2 relatives (6%) as compared to DM2 patients (24%) (odds ratio (OR)=5.24; 95% confidence interval (CI)=1.9-15.8, p<0.0005), but similar to non-diabetic subjects with DM2 relatives (29%) (OR=0.77; CI=0.3-1.7, p=0.4). Logistic regression analysis showed the association of TNF-α -308G/A polymorphism with DM2 family history (OR=5.80; CI=1.77-18.98, p<0.0003).
CONCLUSIONS
Our results suggest that TNF-α -308G/A polymorphism is associated with DM2 family history and is a risk factor for DM2.
目的
我们研究了肿瘤坏死因子-α（TNF-α）启动子基因-308G/A 多态性与 2 型糖尿病（DM2）患者及无 DM2 家族史的非糖尿病个体和有 DM2 家族史的非糖尿病个体之间的可能关联。
方法
我们研究了至少两代中无 DM2 家族史的 87 名非糖尿病个体、48 名有 DM2 家族史的非糖尿病个体和 95 名 DM2 患者。采用 PCR-RFLP 法进行基因分型。
结果
无 DM2 亲属的非糖尿病个体（6%）TNF-α-308G/A 基因型频率明显低于 DM2 患者（24%）（比值比（OR）=5.24；95%置信区间（CI）=1.9-15.8，p<0.0005），但与有 DM2 亲属的非糖尿病个体（29%）相似（OR=0.77；CI=0.3-1.7，p=0.4）。Logistic 回归分析显示 TNF-α-308G/A 多态性与 DM2 家族史有关（OR=5.80；CI=1.77-18.98，p<0.0003）。
结论
我们的结果表明，TNF-α-308G/A 多态性与 DM2 家族史有关，是 DM2 的危险因素。
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