Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine Zhejiang University, 310003, Hangzhou, Zhejiang Province, People's Republic of China.
Immunogenetics. 2012 Mar;64(3):251-8. doi: 10.1007/s00251-011-0580-2. Epub 2011 Oct 21.
The non-classical human leukocyte antigens (HLA)-E, HLA-G, and HLA-F have been shown to modulate immune responses. We examined whether non-classical HLA polymorphisms are associated with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). Fifteen Single-nucleotide polymorphisms (SNPs) in these non-classical class I alleles were investigated by ligase detection reaction. A fragment of 650 bp located in the 3' untranslated region of HLA-G was investigated. Four SNPs (rs17875380, rs41557518, rs114465251, and rs115492845) were associated with altered susceptibility to HBV or HCC, and HLA-F01:04, HLA-G01:05N, and HLA-E*01:01 were associated with hepatitis B or hepatitis B complicated with HCC. Six of 16 designated HLA-E, -G, and -F haplotypes were associated with risk of hepatitis B or HCC. Our study provides healthy reference and detailed analyses of non-classical HLA class Ι polymorphisms that provide insight into immune mechanisms involved in susceptibility to hepatitis B and HCC.
非经典人类白细胞抗原（HLA）-E、HLA-G 和 HLA-F 已被证明可调节免疫反应。我们研究了非经典 HLA 多态性是否与乙型肝炎病毒（HBV）感染和肝细胞癌（HCC）有关。通过连接酶检测反应检测了这些非经典 I 类等位基因中的 15 个单核苷酸多态性（SNP）。研究了 HLA-G 3'非翻译区 650 bp 片段的 SNP。四个 SNP（rs17875380、rs41557518、rs114465251 和 rs115492845）与 HBV 或 HCC 的易感性改变相关，HLA-F01:04、HLA-G01:05N 和 HLA-E*01:01 与乙型肝炎或乙型肝炎合并 HCC 相关。16 个指定的 HLA-E、-G 和 -F 单体型中有 6 个与乙型肝炎或 HCC 的风险相关。我们的研究为非经典 HLA Ⅰ类多态性提供了健康参考和详细分析，为乙型肝炎和 HCC 易感性相关的免疫机制提供了深入了解。
