在活细胞中对多种信号转导活动的协调进行成像。
Imaging the coordination of multiple signalling activities in living cells.
机构信息
Department of Pharmacology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
出版信息
Nat Rev Mol Cell Biol. 2011 Oct 21;12(11):749-56. doi: 10.1038/nrm3212.
Abstract
Cellular signal transduction occurs in complex and redundant interaction networks, which are best understood by simultaneously monitoring the activation dynamics of multiple components. Recent advances in biosensor technology have made it possible to visualize and quantify the activation of multiple network nodes in the same living cell. The precision and scope of this approach has been greatly extended by novel computational approaches (referred to as computational multiplexing) that can reveal relationships between network nodes imaged in separate cells.
摘要
细胞信号转导发生在复杂且冗余的相互作用网络中，通过同时监测多个组件的激活动态，可以最好地理解这些网络。生物传感器技术的最新进展使得在同一个活细胞中可视化和量化多个网络节点的激活成为可能。通过新颖的计算方法（称为计算多路复用），可以揭示在分离的细胞中成像的网络节点之间的关系，从而大大扩展了这种方法的精度和范围。
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