Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, 608 502, India.
Bull Environ Contam Toxicol. 2012 Jan;88(1):30-7. doi: 10.1007/s00128-011-0427-4. Epub 2011 Oct 21.
The 72-h IC(50), 7-d no observable effect concentration (NOEC), low observable effect concentration (LOEC), Chronic values were derived for copper on the growth of marine diatom, Odontella mobiliensis. The effect of copper was also studied on cell morphology, size, nitrate reductase and antioxidant enzymes (Catalase, Superoxide dismutase and peroxidase). The 72-h IC(50) of 298.4 ± 28.3, NOEC of 15.6, LOEC of 29.6 and chronic value of 21.5 μg Cu L(-1) were found in the present study. The chlorophyll a was significantly decreased with increasing concentrations of copper. The length of the cell (apical axis) was extended from 30.14 ± 5.98 μm at control to 71.4 ± 6.29 μm at 574 μg Cu L(-1), the spines were absent at 574 μg L(-1) and the cell structure was entirely damaged at 926 μg Cu L(-1). The antioxidant enzymes viz. Catalase, Peroxidase activities and Melondialdehyde were increased whereas the Nitrate reductase and activity was reduced at 21.5 μg Cu L(-1) during 7 days exposure.
采用 72 小时半抑制浓度（IC50）、7 天无可见效应浓度（NOEC）、低可见效应浓度（LOEC）和慢性值的方法，研究了铜对海洋硅藻 Odontella mobiliensis 生长的影响。还研究了铜对细胞形态、大小、硝酸还原酶和抗氧化酶（过氧化氢酶、超氧化物歧化酶和过氧化物酶）的影响。本研究发现，铜的 72 小时 IC50 为 298.4±28.3μg Cu L(-1)，NOEC 为 15.6μg Cu L(-1)，LOEC 为 29.6μg Cu L(-1)，慢性值为 21.5μg Cu L(-1)。随着铜浓度的增加，叶绿素 a 显著减少。细胞长度（顶端轴）从对照时的 30.14±5.98μm 延长到 574μg Cu L(-1)时的 71.4±6.29μm，574μg Cu L(-1)时无刺，926μg Cu L(-1)时细胞结构完全受损。抗氧化酶（过氧化氢酶、过氧化物酶）活性和丙二醛增加，而硝酸还原酶和活性在 21.5μg Cu L(-1)时降低，暴露 7 天。
