Reiriz S, Cid A, Torres E, Abalde J, Herrero C
Laboratorio de Microbiología, Facultad de Ciencias, Universidad de La Coruña.
Microbiologia. 1994 Sep;10(3):263-72.
Different responses of the marine diatom Phaeodactylum tricornutum (Bohlin) to toxic copper concentrations were investigated. Besides the classical variables applied to toxicity studies in microalgae, such as growth or chlorophyll a content, other variables analyzed by flow cytometry were used. Toxic effects due to copper concentration were observed. Cell density reached in the stationary phase was reduced to 50% in cultures with 20 mg Cu/l, with respect to control cultures without copper. Cell light scatter properties (related to cell volume and intracellular granularity) and chlorophyll a fluorescence of microalgal cells were determined by flow cytometry analysis at the beginning of growth, 1 h after copper exposure, and when cultures reached the stationary phase (72 h). After 1 h of exposure to metal, no differences were observed, but when cultures reached the stationary phase, a gradual increase in the variables analyzed by flow cytometry was observed as the copper concentration increased. The increase in chlorophyll a fluorescence detected by flow cytometry was not correlated with an increase in the cell content of this photopigment, thus indicating an inhibitory effect of copper on photosystem II.
研究了海洋硅藻三角褐指藻（Phaeodactylum tricornutum (Bohlin)）对有毒铜浓度的不同反应。除了应用于微藻毒性研究的经典变量，如生长或叶绿素a含量外，还使用了通过流式细胞术分析的其他变量。观察到了铜浓度引起的毒性效应。与不含铜的对照培养物相比，在含有20 mg Cu/l的培养物中，稳定期达到的细胞密度降低到了50%。在生长开始时、铜暴露1小时后以及培养物达到稳定期（72小时）时，通过流式细胞术分析确定了微藻细胞的光散射特性（与细胞体积和细胞内颗粒度有关）和叶绿素a荧光。暴露于金属1小时后，未观察到差异，但当培养物达到稳定期时，随着铜浓度的增加，通过流式细胞术分析的变量逐渐增加。流式细胞术检测到的叶绿素a荧光增加与这种光合色素的细胞含量增加无关，因此表明铜对光系统II有抑制作用。
