Hao Peng, Wu Yihui, Li Fengjie
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, Jilin Province, China.
Appl Opt. 2011 Oct 1;50(28):5555-8. doi: 10.1364/AO.50.005555.
In this report, gold nanorods (GNRs) were used to enhance the sensitivity of the wavelength-modulated surface plasmon resonance (SPR) biosensor. The GNRs were designed and fabricated through seed-medicated growth and surface activation by a layer of a weak polyelectrolyte, poly(acrylic acid) for the attaching antibody. Rabbit anti-goat IgG was immobilized on GNRs, and sandwich assays were carried out to detect goat IgG using a wavelength-modulated SPR biosensor. The detection sensitivity of the nanorod-conjugated antibody is 25-100 times more sensitive than the SPR biosensor without GNRs. Drastic sensitivity enhancement, owing to the electromagnetic interaction between the nanotag and the sensing film, was maximized using the longitudinal plasmonic resonance of the GNRs. GNRs could significantly enhance the sensitivity of the SPR biosensor, and the maximum enhancement effect can be achieved when the longitudinal SPR peak wavelength of GNRs functionally matches the surface plasmon wavelength.
在本报告中，金纳米棒（GNRs）被用于提高波长调制表面等离子体共振（SPR）生物传感器的灵敏度。通过种子介导生长以及用一层用于附着抗体的弱聚电解质聚丙烯酸进行表面活化，设计并制备了金纳米棒。将兔抗山羊IgG固定在金纳米棒上，并使用波长调制SPR生物传感器进行夹心测定以检测山羊IgG。与不含金纳米棒的SPR生物传感器相比，纳米棒偶联抗体的检测灵敏度高25至100倍。由于纳米标签与传感膜之间的电磁相互作用，利用金纳米棒的纵向等离子体共振使显著的灵敏度增强最大化。金纳米棒可显著提高SPR生物传感器的灵敏度，当金纳米棒的纵向SPR峰值波长与表面等离子体波长在功能上匹配时，可实现最大增强效果。
