Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
Anal Chim Acta. 2011 Jan 10;683(2):242-7. doi: 10.1016/j.aca.2010.10.033. Epub 2010 Oct 31.
An enhanced sensitive biosensor has been developed to detect biological targets by tailoring the localized surface plasmon resonance property of core-shell gold nanorods. In this new concept, a shell layer is produced on gold nanorods by generating a layer of chalcogenide on the gold nanorod surface after attachment of the recognition reagent, namely, goat IgG and antigen of schistosomiasis japonica. The bioactivity of these attached biomolecules is retained and the sensitivity of this biosensor is thus enhanced significantly. The plasmonic properties of the gold nanorods attached with the biomolecules can be adjusted and the plasmon resonance wavelength can be red-shifted up to several hundred nanometers in the visible or near infrared (NIR) region, which is extremely important to biosensing applications. This leads to a lager red-shift in the localized surface plasmon resonance absorption compared to the original gold nanorod-based sensor and hence offers greatly enhanced sensitivity in the detection of schistosomiasis japonica. The human serum infected with schistosomiasis japonica diluted to 1:50,000 (volume ratio, serum/buffer solution) can be detected readily. The technique offers enhanced sensitivity and can be easily extended to other sensing applications based on not only immuno-recognition but also other types of specific reactions.
一种增强型敏感生物传感器已经被开发出来，通过调整核壳金纳米棒的局域表面等离子体共振特性来检测生物靶标。在这个新概念中，通过在金纳米棒表面上生成一层硫属化物，在金纳米棒表面上生成一层硫属化物，在附着识别试剂（即山羊 IgG 和日本血吸虫抗原）后，在金纳米棒上生成一层壳层。这些附着的生物分子的生物活性得以保留，并且生物传感器的灵敏度显著增强。附着有生物分子的金纳米棒的等离子体特性可以被调整，并且等离子体共振波长可以在可见或近红外（NIR）区域中红移多达几百纳米，这对于生物传感应用非常重要。这导致与基于原始金纳米棒的传感器相比，局域表面等离子体共振吸收发生更大的红移，从而在检测日本血吸虫病方面提供了极大的灵敏度增强。可以轻易地检测到稀释至 1:50000（体积比，血清/缓冲溶液）的感染日本血吸虫病的人血清。该技术提供了增强的灵敏度，并且不仅可以容易地扩展到基于免疫识别的其他传感应用，还可以扩展到其他类型的特定反应的传感应用。
