Guo Yan, Li Yikui, Wu Cuiping, Zhao Yihong, Li Yinchao, Liu Xuewu, Wang Naijie, Huang Wenzhe
College of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1879-83.
To observe the anaphylaxis and hemocytolysis of 6 kinds of tween-80 injection from different source.
The Hartley albinism guinea pig was used to carry on the active systematic anaphylaxis (ASA) and the passive cutaneous anaphylaxis (PCA). The in vitro hemolytic experiment and the hemocytolysis was observed by means of spectrophotometry on the domestic rabbit.
The result of ASA and PLA of 6 kinds of tween-80 injection from different source assumed the negative. In observation time, the temolysis rate of 3 kinds of tween-80 injection are more than 5%, while the others are also more than 5% only in the highly concentrated test tube.
Six kinds of tween-80 injection from different source have not caused the immune-mediated anaphylaxis, but it may have hematolysis tendency on intravenous injection. The hemocytolysis of tween-80 may not be entirely caused by the impurities. It is worthy of further study that the physical and chemical properties of the product itself and the undeserved concentration is doubtful whether there is also some internal relations with the generation of hemolytic.
观察6种不同来源的吐温-80注射液的过敏反应及溶血情况。
采用Hartley白化豚鼠进行主动全身过敏反应（ASA）和被动皮肤过敏反应（PCA）。采用分光光度法对家兔进行体外溶血实验并观察溶血情况。
6种不同来源的吐温-80注射液的ASA和PCA结果均为阴性。在观察时间内，3种吐温-80注射液的溶血率超过5%，而其他几种仅在高浓度试管中溶血率超过5%。
6种不同来源的吐温-80注射液未引起免疫介导的过敏反应，但静脉注射时可能有溶血倾向。吐温-80的溶血可能不完全由杂质引起。产品本身的理化性质及不当浓度与溶血发生是否也存在某种内在联系值得进一步研究。
