清开灵注射液对体外补体及RBL-2H3细胞的作用
[Effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto].
作者信息
Chen Lijing, Liao Guoping, Wang Yan, He Shuai, Zhang Zhongyi
机构信息
Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1884-8.
OBJECTIVE
To investigate the effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto.
METHOD
The mixture of human serums and QKLI were incubated for 30 min in vitro and then the content of SC5 b-9 in the mixture was determined by ELISA. RBL-2 H3 cells were cultured and treated by QKLI. Beta-heosaminidase release rate was measured by coloration method. The content of histamine in supernatant was tested by ELISA.
RESULT
The QKLI can reduce the content of SC5 b-9 (P<0.05) and promote the release of beta-heosaminidase and histamine significantly (P<0.05).
CONCLUSION
QKLI didn't induce the complement activation, but induced the release of beta-heosaminidase and histamine directly. Therefore, the clinical adverse reactions of QKLI in clinic may be pseudoallergy which had no relation with the activation of complement system.
目的
体外研究清开灵注射液（QKLI）对补体及RBL - 2H3细胞的影响。
方法
人血清与QKLI混合液在体外孵育30分钟，然后用ELISA法测定混合液中SC5b - 9的含量。培养RBL - 2H3细胞并用QKLI处理。用比色法测定β - 己糖胺酶释放率。用ELISA法检测上清液中组胺的含量。
结果
QKLI可降低SC5b - 9的含量（P<0.05），并显著促进β - 己糖胺酶和组胺的释放（P<0.05）。
结论
QKLI不诱导补体激活，但直接诱导β - 己糖胺酶和组胺的释放。因此，QKLI在临床上的不良反应可能是假性过敏，与补体系统激活无关。
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