Zhong Guangwei, Hu Jianjun, Chen Zeqi, Luo Yanhong, Zhang Ying, Yin Yaohui, Li Wei, Yi Zhenjia, Chen Guolin
Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Jan;35(1):70-6. doi: 10.3969/j.issn.1672-7347.2010.01.010.
To explore the molecular mechanism of the formulae for calming the liver and suppressing YANG in migraine rat model with syndrome of hyperactivity of the liver-YANG.
A rat model of migraine with hyperactivity of liver-YANG was established through electrical trigeminal ganglion stimulation and syndrome of oral administration of Fuzi decoction. The total proteins of the lymphocyte in the rats were separated by immobilized pH gradient-based 2-dimensional gel electrophoresis (2-DE), and the 2-DE image was analyzed by PDQuest 7.0 software. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) and the SWISS-PORT and MSDB database were used to identify differential proteins.
The formulae for calming the liver and suppressing YANG could also improve headache. Well-resolution and reproducible 2-DE patterns of rat lymphocyte from normal, model, and therapy tissues were obtained. Eleven of the total 13 differential protein spots were successfully identified by MALDI-TOF-MS. These proteins were alcohol dehydrogenase 3 (ADH3), glycogen phosphorylase, ATP synthase D chain, annexin-3, ubiquitin, neutrophil defensin 4 precursor, melanoma-associated antigen E2, heat shock protein-27, annexin-A1, peroxirdoxin-II, MU class glutathione S-transferase (Fragment)(GSH).
Differences occur in the expression of lymphocyte proteins in migraine rats with syndrome of hyperactivity of liver-YANG after treatment with the formulae for calming the liver and suppressing YANG, and the 11 identified protein spots may be associated with its mechanism.
探讨平肝潜阳方对肝阳上亢型偏头痛大鼠模型的分子作用机制。
采用电刺激三叉神经节结合灌服附子汤造模，制备肝阳上亢型偏头痛大鼠模型。运用基于固定化pH梯度的双向凝胶电泳（2-DE）技术分离大鼠淋巴细胞总蛋白，并用PDQuest 7.0软件分析2-DE图像。采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）技术，并结合SWISS-PORT和MSDB数据库对差异蛋白进行鉴定。
平肝潜阳方能够改善偏头痛大鼠的头痛症状。获得了正常组、模型组和治疗组大鼠淋巴细胞分辨率高且重复性好的2-DE图谱。通过MALDI-TOF-MS成功鉴定出13个差异蛋白点中的11个。这些蛋白分别为乙醇脱氢酶3（ADH3）、糖原磷酸化酶、ATP合酶D链、膜联蛋白-3、泛素、中性粒细胞防御素4前体、黑色素瘤相关抗原E2、热休克蛋白-27、膜联蛋白-A1、过氧化物酶-II、MU类谷胱甘肽S-转移酶（片段）（GSH）。
平肝潜阳方治疗后，肝阳上亢型偏头痛大鼠淋巴细胞蛋白表达出现差异，所鉴定出的11个蛋白点可能与其作用机制相关。
