Center for Advanced Study in Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Nat Prod Res. 2012;26(12):1161-6. doi: 10.1080/14786419.2011.562205. Epub 2011 Oct 24.
The objective of this study was to evaluate the anti-diabetic potential of three mangrove plants, Rhizophora mucronata, Rhizophora apiculata and Rhizophora annamalayana, and to detect the presence of their insulin-like protein. The in vivo anti-diabetic experiment was done on male albino Wister rats. Oral administration of 60 mg kg(-1) leaf powder extract of the three different mangrove plants for 30 days modulated the parameters such as blood glucose, plasma insulin, body weight, total haemoglobin, glycosylated haemoglobin, liver glycogen, plasma and tissue lipids, cholesterol, triglycerides, free fatty acids and phospholipids to normal levels in the alloxan-induced diabetic rats. The anti-diabetic activity of R. apiculata was more pronounced than that of the other mangrove extracts, but it was on a par with the commercial drug glibenclamide. The presence of an insulin-like protein in the mangrove extracts was detected by SDS-PAGE analysis and confirmed through ELISA. Hence, the anti-diabetic activity and the presence of an insulin-like protein in Rhizophora species were proved scientifically.
本研究旨在评估三种红树林植物——红树（Rhizophora mucronata）、角果木（Rhizophora apiculata）和海桑（Rhizophora annamalayana）的抗糖尿病潜力，并检测其胰岛素样蛋白的存在。在雄性白化 Wistar 大鼠中进行了体内抗糖尿病实验。三种不同红树林植物的叶片粉末提取物以 60mg/kg 的剂量经口给药 30 天，可调节参数，如血糖、血浆胰岛素、体重、总血红蛋白、糖化血红蛋白、肝糖原、血浆和组织脂质、胆固醇、甘油三酯、游离脂肪酸和磷脂，使在链脲佐菌素诱导的糖尿病大鼠中恢复到正常水平。角果木提取物的抗糖尿病活性比其他红树林提取物更为明显，但与商业药物格列本脲相当。通过 SDS-PAGE 分析和 ELISA 证实了红树林提取物中存在胰岛素样蛋白。因此，从科学上证明了红树属植物的抗糖尿病活性和存在胰岛素样蛋白。
