Friis Morten, Martin-Bertelsen Tomas, Friis-Hansen Lennart, Winther Ole, Henao Ricardo, Sørensen Mads Sølvsten, Qvortrup Klaus
Department of Otolaryngology, Head & Neck Surgery, Rigshospitalet, Copenhagen, Denmark.
Acta Otolaryngol. 2011 Dec;131(12):1257-63. doi: 10.3109/00016489.2011.616910. Epub 2011 Oct 23.
The endolymphatic sac is part of the membranous inner ear and is thought to play a role in the fluid homeostasis and immune defense of the inner ear; however, the exact function of the endolymphatic sac is not fully known. Many of the detected mRNAs in this study suggest that the endolymphatic sac has multiple and diverse functions in the inner ear.
The objective of this study was to provide a comprehensive review of the genes expressed in the endolymphatic sac in the rat and perform a functional characterization based on measured mRNA abundance.
Microarray technology was used to investigate the gene expression of the endolymphatic sac with the surrounding dura. Characteristic and novel endolymphatic sac genes were determined by comparing with expressions in pure dura.
In all, 463 genes were identified specific for the endolymphatic sac. Functional annotation clustering revealed 29 functional clusters.
内淋巴囊是膜迷路的一部分，被认为在内耳的液体稳态和免疫防御中发挥作用；然而，内淋巴囊的确切功能尚未完全明确。本研究中检测到的许多mRNA表明，内淋巴囊在内耳具有多种不同的功能。
本研究的目的是全面综述大鼠内淋巴囊中表达的基因，并根据测得的mRNA丰度进行功能表征。
采用微阵列技术研究内淋巴囊与周围硬脑膜的基因表达。通过与纯硬脑膜中的表达进行比较，确定内淋巴囊的特征性和新基因。
共鉴定出463个内淋巴囊特异性基因。功能注释聚类显示有29个功能簇。
