Barthel L K, Raymond P A
Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor 48109-0616.
J Histochem Cytochem. 1990 Sep;38(9):1383-8. doi: 10.1177/38.9.2201738.
The following describes a modified technique for obtaining 3-microns sections for light microscopic level immunocytochemistry. By combining 20% sucrose with Tissue-Tek OCT embedding compound in a ratio of 2:1, we produced a block that was suitable for cutting 3-microns sections on a conventional cryostat. The 3-microns sections were dramatically improved compared with 10-microns sections cut from tissue embedded in OCT alone, when viewed with both differential interference contrast microscopy (Nomarski optics) and indirect immunofluorescence. The method is simple, uses materials already available, and does not require training in a new technique.
以下介绍一种用于光镜水平免疫细胞化学的获取3微米切片的改良技术。通过将20%的蔗糖与Tissue-Tek OCT包埋剂按2:1的比例混合，我们制作出了一个适合在传统低温恒温切片机上切割3微米切片的组织块。与仅用OCT包埋的组织切割的10微米切片相比，在微分干涉相差显微镜（诺马斯基光学）和间接免疫荧光观察下，3微米切片有了显著改善。该方法简单，使用现有的材料，且不需要新的技术培训。
