Department of Chemistry, Zabol University, Zabol, Iran.
Nat Prod Res. 2012;26(20):1926-30. doi: 10.1080/14786419.2011.616506. Epub 2011 Oct 21.
Essential oil of Diplotaenia cachrydifolia cultivated in Iran was obtained by supercritical fluid extraction (SFE) method. The oils were analysed by capillary gas chromatography using flame ionisation and mass spectrometric detections. The compounds were identified according to their retention indices and mass spectra (EI, 70 eV). The effects of different parameters, such as pressure, temperature, modifier volume and extraction times (dynamic and static), on the SFE were inspected by a fractional factorial design (2(5-2)) to identify the significant parameters and their interaction. It showed that static and dynamic times had no effect on the extraction. Finally, a Box-Behnken design was applied to obtain the optimum condition of the significant parameters. The optimal condition was obtained as 30.2 MPa for pressure, 65.6°C for temperature and 258.4 µL for modifier volume. The main components that were extracted with SFE were dillapiole (35.1%), limonene (33.5%) and α-calacorene (25.5%).
伊朗种植的 Diplotaenia cachrydifolia 的精油通过超临界流体萃取（SFE）法获得。使用火焰离子化和质谱检测的毛细管气相色谱法对油进行分析。根据保留指数和质谱（EI，70eV）确定化合物的身份。通过 2(5-2)部分因子设计检验不同参数（如压力、温度、改性剂体积和提取时间（动态和静态））对 SFE 的影响，以确定显著参数及其相互作用。结果表明，静态和动态时间对萃取没有影响。最后，应用 Box-Behnken 设计获得显著参数的最佳条件。最佳条件为压力 30.2MPa、温度 65.6°C 和改性剂体积 258.4µL。用 SFE 提取的主要成分是 dillapiole（35.1%）、柠檬烯（33.5%）和α- calacorene（25.5%）。
