Department of Plant Pathology, Engineering Center of Bioresource Pesticide in Jiangsu Province, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, College of Plant Protection, Nanjing Agricultural University, China.
Lett Appl Microbiol. 2012 Jan;54(1):10-7. doi: 10.1111/j.1472-765X.2011.03165.x. Epub 2011 Nov 18.
The effects of different freeze-drying protective agents on the viabilities of biocontrol strains Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40 were investigated.
Several concentrations of protective and rehydration media were tested to improve the survival of biocontrol agents after freeze-drying. The subsequent survival rates during storage and rehydration media of freeze-dried biocontrol strains were also examined.
The results indicated that cellobiose (5%) and d-galactose (5%) gave maximum viability of strains Bu. vietnamiensis B418 and P. agglomerans 2Re40 (98 and 54·3% respectively) while the perfect one (100%) of strain B. cereus AR156 was obtained with sucrose (5%) during freeze-drying, and the highest survival of the three strains was reached when they were rehydrated with 10% nonfat skim milk. In the following storage, the survival rates showed that B. cereus AR156 could still reach 50% after 12 months.
This study showed that freeze-drying could be used to stabilize cells of these three biocontrol strains. Further studies should focus on the scale-up possibilities and formulation development.
研究不同冷冻干燥保护剂对生防菌株蜡样芽胞杆菌 AR156、越橘生黑单胞菌 B418 和成团泛菌 2Re40 活力的影响。
测试了几种保护剂和复水介质的浓度，以提高生防剂在冷冻干燥后的存活率。还研究了冷冻干燥生防菌株在储存和复水介质中的后续存活率。
结果表明，在冷冻干燥时，纤维二糖（5%）和 D-半乳糖（5%）使越橘生黑单胞菌 B418 和成团泛菌 2Re40 的活力达到最大值（分别为 98%和 54.3%），而蜡样芽胞杆菌 AR156 的最佳活力（100%）是用 5%蔗糖获得的，当用 10%无脂脱脂乳复水时，三株菌的存活率最高。在随后的储存中，存活率表明，蜡样芽胞杆菌 AR156 在 12 个月后仍能达到 50%。
本研究表明，冷冻干燥可用于稳定这三种生防菌株的细胞。进一步的研究应集中在扩大规模的可能性和配方开发上。
