Chege G M, Beier J C
Kenya Medical Research Institute, Nairobi.
J Med Entomol. 1990 Jul;27(4):454-8. doi: 10.1093/jmedent/27.4.454.
The effect of the malarial parasite, Plasmodium falciparum Welch, on the daily survival rates and longevity of Anopheles gambiae Giles sensu lato and Anopheles funestus Giles was determined for wild-caught, naturally infected females from western Kenya. Mosquitoes were collected inside houses and held in cages until death, after which they were assayed for P. falciparum circumsporozoite protein by an enzyme-linked immunosorbent assay (ELISA). Survival rates of field populations determined by parity rates were significantly higher than survival rates estimated by regression for Anopheles dying in cages. Overall, An. gambiae s.l. had a significantly higher daily mortality rate (means = 17.5%) than An. funestrus (means = 13.2%). P. falciparum ELISA infection rates, which were higher for An. gambiae s.l. (means = 19.8%; n = 1,221) than for An. funestus (means = 11.9%; n = 1,128), did not increase as a function of time for caged Anopheles. For An. gambiae s.l., there was a significant negative correlation between holding time and P. falciparum ELISA absorbance, suggesting that detectable circumsporozoite protein and perhaps the number of sporozoites may decrease with time in the vector. In western Kenya, an area where Anopheles populations often have extremely high malaria infection rates. Plasmodium infections did not reduce vector survivorship.
对于从肯尼亚西部野外捕获的、自然感染的雌性冈比亚按蚊广义种（Anopheles gambiae Giles sensu lato）和费氏按蚊（Anopheles funestus Giles），测定了疟原虫恶性疟原虫（Plasmodium falciparum Welch）对其每日存活率和寿命的影响。蚊子在房屋内收集并置于笼中直至死亡，之后通过酶联免疫吸附测定法（ELISA）检测其恶性疟原虫环子孢子蛋白。通过产卵率确定的野外种群存活率显著高于通过回归估计的笼中死亡的冈比亚按蚊的存活率。总体而言，冈比亚按蚊广义种的每日死亡率（均值 = 17.5%）显著高于费氏按蚊（均值 = 1 .2%）。恶性疟原虫ELISA感染率在冈比亚按蚊广义种中（均值 = 19.8%；n = 1221）高于费氏按蚊（均值 = 11.9%；n = 1128），对于笼养的按蚊，感染率并未随时间增加。对于冈比亚按蚊广义种，饲养时间与恶性疟原虫ELISA吸光度之间存在显著负相关,这表明在媒介中，可检测到的环子孢子蛋白以及可能的子孢子数量可能会随时间减少。在肯尼亚西部这个按蚊种群疟疾感染率通常极高的地区，疟原虫感染并未降低媒介的存活率。
