Solae, LLC, 4300 Duncan Avenue, St. Louis, Missouri 63110, United States.
J Agric Food Chem. 2011 Dec 14;59(23):12707-12. doi: 10.1021/jf203220v. Epub 2011 Nov 16.
Protein quality, as determined by the PDCAAS method, is a measure of a protein's ability to provide adequate levels of essential amino acids for human needs. PDCAAS is calculated using an amino acid profile and true digestibility of a food protein. Soy protein is recognized as a high quality plant protein, but published PDCAAS values may vary based on the soy protein ingredient as well as the reproducibility and accuracy of the testing methods. Comparison of PDCAAS values for four differently processed soy ingredients, including three isolated soy proteins (ISP) and one soy protein concentrate (SPC), was made using two different laboratories with evaluation of the impact of the reproducibility and accuracy of amino acid profiles. PDCAAS calculations, using amino acid values from one laboratory, yielded a truncated PDCAAS of 1.00 for all four ingredients, while a second laboratory provided statistically significantly lower scores (0.95-1.00). We conclude that analytical method error can be a significant contributor to PDCAAS differences and can be mitigated by the application of amino acid nitrogen recovery correction factors.
蛋白质质量,由 PDCAAS 方法决定,是衡量蛋白质为人体提供充足必需氨基酸能力的指标。PDCAAS 通过氨基酸组成和食物蛋白质的真实消化率计算得出。大豆蛋白被认为是一种高质量的植物蛋白,但根据大豆蛋白成分以及测试方法的可重复性和准确性,发表的 PDCAAS 值可能会有所不同。使用两个不同的实验室对四种不同加工的大豆成分(包括三种分离大豆蛋白[ISP]和一种大豆浓缩蛋白[SPC])进行了 PDCAAS 值比较,评估了氨基酸组成的可重复性和准确性的影响。使用一个实验室的氨基酸值进行 PDCAAS 计算,所有四种成分的截断 PDCAAS 值均为 1.00,而第二个实验室提供的分数明显较低(0.95-1.00)。我们得出结论,分析方法误差可能是 PDCAAS 差异的一个重要因素,通过应用氨基酸氮回收校正因子可以减轻这种影响。