Mathai John K, Liu Yanhong, Stein Hans H
1Division of Nutritional Sciences,University of Illinois,Urbana,IL 61801,USA.
2Department of Animal Sciences,University of Illinois,Urbana,IL 61801,USA.
Br J Nutr. 2017 Feb;117(4):490-499. doi: 10.1017/S0007114517000125.
An experiment was conducted to compare values for digestible indispensable amino acid scores (DIAAS) for four animal proteins and four plant proteins with values calculated as recommended for protein digestibility-corrected amino acid scores (PDCAAS), but determined in pigs instead of in rats. Values for standardised total tract digestibility (STTD) of crude protein (CP) and standardised ileal digestibility (SID) of amino acids (AA) were calculated for whey protein isolate (WPI), whey protein concentrate (WPC), milk protein concentrate (MPC), skimmed milk powder (SMP), pea protein concentrate (PPC), soya protein isolate (SPI), soya flour and whole-grain wheat. The PDCAAS-like values were calculated using the STTD of CP to estimate AA digestibility and values for DIAAS were calculated from values for SID of AA. Results indicated that values for SID of most indispensable AA in WPI, WPC and MPC were greater (P<0·05) than for SMP, PPC, SPI, soya flour and wheat. With the exception of arginine and tryptophan, the SID of all indispensable AA in SPI was greater (P<0·05) than in soya flour, and with the exception of threonine, the SID of all indispensable AA in wheat was less (P<0·05) than in all other ingredients. If the same scoring pattern for children between 6 and 36 months was used to calculate PDCAAS-like values and DIAAS, PDCAAS-like values were greater (P<0·05) than DIAAS values for SMP, PPC, SPI, soya flour and wheat indicating that PDCAAS-like values estimated in pigs may overestimate the quality of these proteins.
进行了一项实验,比较四种动物蛋白和四种植物蛋白的可消化必需氨基酸评分(DIAAS)值与按照蛋白质消化率校正氨基酸评分(PDCAAS)建议计算的值,但这些值是在猪而非大鼠身上测定的。计算了乳清蛋白分离物(WPI)、乳清蛋白浓缩物(WPC)、乳蛋白浓缩物(MPC)、脱脂奶粉(SMP)、豌豆蛋白浓缩物(PPC)、大豆蛋白分离物(SPI)、大豆粉和全麦的粗蛋白(CP)的标准化全肠道消化率(STTD)以及氨基酸(AA)的标准化回肠消化率(SID)。使用CP的STTD来估计AA消化率计算出类PDCAAS值,并根据AA的SID值计算DIAAS值。结果表明,WPI、WPC和MPC中大多数必需AA的SID值高于(P<0·05)SMP、PPC、SPI、大豆粉和小麦。除精氨酸和色氨酸外,SPI中所有必需AA的SID值高于(P<0·05)大豆粉,除苏氨酸外,小麦中所有必需AA的SID值低于(P<0·05)所有其他成分。如果使用相同的6至36个月儿童评分模式来计算类PDCAAS值和DIAAS,则SMP、PPC、SPI、大豆粉和小麦的类PDCAAS值高于(P<0·05)DIAAS值,这表明在猪身上估计的类PDCAAS值可能高估了这些蛋白质的质量。