胆囊良恶性病变中 MK-1 和 RegIV 的表达及其临床病理意义。

Expression of MK-1 and RegIV and its clinicopathological significances in the benign and malignant lesions of gallbladder.

机构信息

Research laboratory of Hepatobiliary Diseases, Second Xiangya Hospital of Central South University, Changsha 410011, China.

出版信息

Diagn Pathol. 2011 Oct 21;6:100. doi: 10.1186/1746-1596-6-100.

DOI:10.1186/1746-1596-6-100

PMID:22018336

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3225305/

Abstract

BACKGROUND

To study the expression of MK-1 and RegIV and to detect their pathological significances in benign and malignant lesions of gallbladder.

METHODS

The expression of MK-1 and RegIV was detected by immunohistochemical method in paraffin-embedded sections of surgical resected specimens from gallbladder adenocarcinoma (n = 108), peritumoral tissues (n = 46), adenomatous polyp (n = 15), and chronic cholecystitis (n = 35).

RESULTS

The positive rate of MK-1 or RegIV expression was significantly higher in gallbladder adenocarcinoma than that in peritumoral tissues (χ(2)(MK-1) = 18.76, P < 0.01; χ(2)(RegIV) = 9.92, P < 0.01), denomatous polyp (χ(2)(MK-1) = 9.49, P < 0.01; χ(2)(RegIV) = 8.59, P < 0.01) and chronic cholecystitis (χ(2)MK-1 = 24.11, P < 0.01; χ(2)(RegIV) = 19.24, P < 0.01). The positive cases of MK-1 and/or RegIV in the benign lesions showed moderately- or severe-atypical hyperplasia of gallbladder epitheli. The positive rates of MK-1 were significantly higher in the cases of well-differentiated adenocarcinoma, no-metastasis of lymph node, and no-invasiveness of regional tissues than those in the ones of differentiated adenocarcinoma, metastasis of lymph node, and invasiveness of regional tissues in gallbladder adenocarcinoma (P < 0.05 or P < 0.01). On the contrary, the positive rates of RegIV were significantly lower in the cases of well-differentiated adenocarcinoma, no-metastasis of lymph node, and no-invasiveness of regional tissues than those in the ones of differentiated adenocarcinoma, metastasis of lymph node, and invasiveness of regional tissues in gallbladder adenocarcinoma (P < 0.05 or P < 0.01). Univariate Kaplan-Meier analysis showed that decreased expression of MK-1 (P = 0.09) or increased expression of RegIV (P = 0.003) was associated with decreased overall survival. Multivariate Cox regression analysis showed that decreased expression of MK-1 (P = 0.033) and increased expression of RegIV (P = 0.008) was an independent prognostic predictor in gallbladder adenocarcinoma.

CONCLUSIONS

The expression of MK-1 and/or RegIV might be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma.

摘要

背景

研究 MK-1 和 RegIV 的表达，并检测其在胆囊良恶性病变中的病理意义。

方法

采用免疫组织化学方法检测 108 例胆囊腺癌、46 例癌旁组织、15 例腺瘤性息肉和 35 例慢性胆囊炎手术切除标本石蜡切片中 MK-1 和 RegIV 的表达。

结果

MK-1 或 RegIV 的阳性率在胆囊腺癌中明显高于癌旁组织（χ(2)(MK-1) = 18.76，P < 0.01；χ(2)(RegIV) = 9.92，P < 0.01）、腺瘤性息肉（χ(2)(MK-1) = 9.49，P < 0.01；χ(2)(RegIV) = 8.59，P < 0.01）和慢性胆囊炎（χ(2)MK-1 = 24.11，P < 0.01；χ(2)(RegIV) = 19.24，P < 0.01）。良性病变中 MK-1 和/或 RegIV 阳性病例表现为胆囊上皮中度或重度不典型增生。MK-1 在分化良好的腺癌、无淋巴结转移、无区域性组织浸润病例中的阳性率明显高于分化差的腺癌、淋巴结转移和区域性组织浸润病例（P < 0.05 或 P < 0.01）。相反，RegIV 在分化良好的腺癌、无淋巴结转移、无区域性组织浸润病例中的阳性率明显低于分化差的腺癌、淋巴结转移和区域性组织浸润病例（P < 0.05 或 P < 0.01）。单因素 Kaplan-Meier 分析表明，MK-1 表达降低（P = 0.09）或 RegIV 表达升高（P = 0.003）与总生存时间降低相关。多因素 Cox 回归分析表明，MK-1 表达降低（P = 0.033）和 RegIV 表达升高（P = 0.008）是胆囊腺癌的独立预后预测因子。