Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, HeilongjiangProvince, China.
J Chromatogr A. 2011 Dec 2;1218(48):8573-80. doi: 10.1016/j.chroma.2011.09.075. Epub 2011 Oct 5.
Ionic liquid-based microwave-assisted extraction (ILMAE) has been successfully applied in extracting essential oil and four kinds of biphenyl cyclooctene lignans from Schisandra chinensis Baill. 0.25 M 1-lauryl-3-methylimidazolium bromide ionic liquid is selected as solvent. The optimum parameters of dealing with 25.0 g sample are 385 W irradiation power, 40 min microwave extraction time and 1:12 solid-liquid ratio. The yields of essential oil and lignans are 12.12±0.37 ml/kg and 250.2±38.2 mg/kg under the optimum conditions. The composition of the essential oil extracted by hydro-distillation, steam-distillation and ILMAE is analyzed by GC-MS. With ILMAE method, the energy consumption time has not only been shortened to 40 min (hydro-distillation 3.0 h for extracting essential oil and reflux extraction 4.0 h for extracting lignans, respectively), but also the extraction efficiency has been improved (extraction of lignans and distillation of essential oil at the same time) and reduces the environmental pollution. S. chinensis materials treated by different methods are observed by scanning electronic microscopy. Micrographs provide more evidence to prove that ILMAE is a better and faster method. The experimental results also indicate that ILMAE is a simple and efficient technique for sample preparation.
离子液体辅助微波萃取（ILMAE）已成功应用于从五味子中提取挥发油和四种联苯环辛烯木脂素。选择 0.25 M 1-月桂基-3-甲基咪唑溴离子液体作为溶剂。处理 25.0 g 样品的最佳参数为 385 W 辐射功率、40 min 微波提取时间和 1:12 的固液比。在最佳条件下，挥发油和木脂素的产率分别为 12.12±0.37 ml/kg 和 250.2±38.2 mg/kg。通过 GC-MS 分析水蒸气蒸馏、水蒸汽蒸馏和 ILMAE 提取的挥发油的组成。采用 ILMAE 方法，不仅将提取挥发油的时间从 3.0 小时（水蒸汽蒸馏）缩短到 40 分钟，同时也提高了提取效率（同时提取木脂素和蒸馏挥发油），并减少了环境污染。通过不同方法处理的五味子材料通过扫描电子显微镜进行观察。显微照片提供了更多的证据来证明 ILMAE 是一种更好、更快的方法。实验结果还表明，ILMAE 是一种简单高效的样品制备技术。
