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SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus.

作者信息

Swanson M S, Carlson M, Winston F

机构信息

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.

出版信息

Mol Cell Biol. 1990 Sep;10(9):4935-41. doi: 10.1128/mcb.10.9.4935-4941.1990.

DOI:10.1128/mcb.10.9.4935-4941.1990
PMID:2201908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361114/
Abstract

SPT6 is an essential gene of Saccharomyces cerevisiae that appears to play a role in transcription. Mutations in the SPT6 (SSN20, CRE2) gene suppress delta insertion mutations in the 5' regions of HIS4 and LYS2 and mutations in cis- and/or trans-acting elements that are required for expression of SUC2 and ADH2. We report here that SPT6 encodes a 170-kilodalton highly charged protein with an extremely acidic amino terminus. By use of an epitope-tagged SPT6 protein, we have determined by indirect immunofluorescence that the SPT6 protein is located in the nucleus.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f5d/361114/a3a385e287b6/molcellb00045-0512-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f5d/361114/df302bf96ecc/molcellb00045-0510-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f5d/361114/401355fd1ea7/molcellb00045-0511-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f5d/361114/a3a385e287b6/molcellb00045-0512-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f5d/361114/df302bf96ecc/molcellb00045-0510-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f5d/361114/401355fd1ea7/molcellb00045-0511-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f5d/361114/a3a385e287b6/molcellb00045-0512-a.jpg

相似文献

1
SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus.
Mol Cell Biol. 1990 Sep;10(9):4935-41. doi: 10.1128/mcb.10.9.4935-4941.1990.
2
SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat.
Mol Cell Biol. 1991 Jun;11(6):3009-19. doi: 10.1128/mcb.11.6.3009-3019.1991.
3
The SPT6 gene is essential for growth and is required for delta-mediated transcription in Saccharomyces cerevisiae.SPT6基因对酿酒酵母的生长至关重要，且是δ介导转录所必需的。
Mol Cell Biol. 1987 Feb;7(2):679-86. doi: 10.1128/mcb.7.2.679-686.1987.
4
SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae.SPT4、SPT5和SPT6的相互作用：对酿酒酵母转录和生存能力的影响
Genetics. 1992 Oct;132(2):325-36. doi: 10.1093/genetics/132.2.325.
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The yeast CCR4 protein is neither regulated by nor associated with the SPT6 and SPT10 proteins and forms a functionally distinct complex from that of the SNF/SWI transcription factors.酵母CCR4蛋白既不受SPT6和SPT10蛋白的调控，也不与之相关联，并且形成了一种与SNF/SWI转录因子功能不同的复合物。
Genetics. 1994 Dec;138(4):1005-13. doi: 10.1093/genetics/138.4.1005.
6
Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae.在酿酒酵母中，Spt4、Spt5和Spt6控制RNA聚合酶II转录延伸的证据。
Genes Dev. 1998 Feb 1;12(3):357-69. doi: 10.1101/gad.12.3.357.
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Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiae.酿酒酵母中激活UAS2依赖性ADH2表达的基因的分离与鉴定。
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8
Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae.酿酒酵母中对转录起始很重要的基因SPT4的分子和遗传特征分析
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emb-5, a gene required for the correct timing of gut precursor cell division during gastrulation in Caenorhabditis elegans, encodes a protein similar to the yeast nuclear protein SPT6.emb-5是秀丽隐杆线虫原肠胚形成过程中肠道前体细胞分裂正确时间所需的一个基因，它编码一种与酵母核蛋白SPT6相似的蛋白质。
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The Saccharomyces cerevisiae SPT8 gene encodes a very acidic protein that is functionally related to SPT3 and TATA-binding protein.酿酒酵母的SPT8基因编码一种酸性很强的蛋白质，该蛋白质在功能上与SPT3及TATA结合蛋白相关。
Genetics. 1994 Jul;137(3):647-57. doi: 10.1093/genetics/137.3.647.

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Comparative transcriptome analysis reveals candidate gene for flowering time QTL HvHeading in barley.比较转录组分析揭示了大麦开花时间QTL HvHeading的候选基因。
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The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stability.

本文引用的文献

1
High-sensitivity sequence analysis of proteins recovered from sodium dodecyl sulfate gels.从十二烷基硫酸钠凝胶中回收的蛋白质的高灵敏度序列分析。
Methods Enzymol. 1983;91:450-5. doi: 10.1016/s0076-6879(83)91042-x.
2
Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae.影响酿酒酵母中葡萄糖阻遏对SUC2基因表达调控的基因。
Genetics. 1984 Dec;108(4):845-58. doi: 10.1093/genetics/108.4.845.
3
Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
保守的组蛋白伴侣 Spt6 强烈需要 DNA 复制和基因组稳定性。
Cell Rep. 2023 Mar 28;42(3):112264. doi: 10.1016/j.celrep.2023.112264. Epub 2023 Mar 15.
4
Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro.酵母 Spt6 在体外读取 RNA 聚合酶 II C 末端结构域的多种磷酸化模式。
J Mol Biol. 2020 Jun 26;432(14):4092-4107. doi: 10.1016/j.jmb.2020.05.007. Epub 2020 May 19.
5
Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality.遗传消融与 Spt6（Iws1）相互作用导致早期胚胎致死。
PLoS One. 2018 Sep 12;13(9):e0201030. doi: 10.1371/journal.pone.0201030. eCollection 2018.
6
Spt6 Association with RNA Polymerase II Directs mRNA Turnover During Transcription.Spt6 与 RNA 聚合酶 II 的结合在转录过程中指导 mRNA 的周转。
Mol Cell. 2018 Jun 21;70(6):1054-1066.e4. doi: 10.1016/j.molcel.2018.05.020.
7
Shaping the cellular landscape with Set2/SETD2 methylation.通过Set2/SETD2甲基化塑造细胞格局。
Cell Mol Life Sci. 2017 Sep;74(18):3317-3334. doi: 10.1007/s00018-017-2517-x. Epub 2017 Apr 6.
8
A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation.一个前馈回路由 Spt6、Ctk1 和 PAF 组成，调节 Pol II CTD 磷酸化和转录延伸。
Nucleic Acids Res. 2014 Jan;42(2):870-81. doi: 10.1093/nar/gkt1003. Epub 2013 Oct 25.
9
Spt6 is required for heterochromatic silencing in the fission yeast Schizosaccharomyces pombe.Spt6 对于裂殖酵母 Schizosaccharomyces pombe 中的异染色质沉默是必需的。
Mol Cell Biol. 2011 Oct;31(20):4193-204. doi: 10.1128/MCB.05568-11. Epub 2011 Aug 15.
10
A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II C-terminal repeat domain (CTD).转录延伸因子 Spt6 中的串联 SH2 结构域结合磷酸化 RNA 聚合酶 II C 末端重复结构域（CTD）。
J Biol Chem. 2010 Dec 31;285(53):41597-603. doi: 10.1074/jbc.M110.144568. Epub 2010 Oct 6.
酿酒酵母细胞周期中微管蛋白和肌动蛋白的结构重排。
J Cell Biol. 1984 Mar;98(3):922-33. doi: 10.1083/jcb.98.3.922.
4
Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.影响酿酒酵母HIS4基因Ty介导表达的突变。
Genetics. 1984 Jun;107(2):179-97. doi: 10.1093/genetics/107.2.179.
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Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.利用寡脱氧核苷酸定向诱变构建改良的M13载体。
Gene. 1983 Dec;26(1):101-6. doi: 10.1016/0378-1119(83)90040-9.
6
Targeting of E. coli beta-galactosidase to the nucleus in yeast.大肠杆菌β-半乳糖苷酶在酵母中靶向细胞核。
Cell. 1984 Apr;36(4):1057-65. doi: 10.1016/0092-8674(84)90055-2.
7
Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine.口蹄疫克隆病毒蛋白疫苗：牛和猪的反应
Science. 1981 Dec 4;214(4525):1125-9. doi: 10.1126/science.6272395.
8
Structure of transcribing chromatin.转录染色质的结构
Prog Nucleic Acid Res Mol Biol. 1980;24:1-55. doi: 10.1016/s0079-6603(08)60670-4.
9
Generation of protein-reactive antibodies by short peptides is an event of high frequency: implications for the structural basis of immune recognition.短肽产生蛋白质反应性抗体是一个高频事件：对免疫识别结构基础的启示。
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4949-53. doi: 10.1073/pnas.80.16.4949.
10
A complementation analysis of the restriction and modification of DNA in Escherichia coli.大肠杆菌中DNA限制与修饰的互补分析。
J Mol Biol. 1969 May 14;41(3):459-72. doi: 10.1016/0022-2836(69)90288-5.