影响酿酒酵母HIS4基因Ty介导表达的突变。
Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.
作者信息
Winston F, Chaleff D T, Valent B, Fink G R
出版信息
Genetics. 1984 Jun;107(2):179-97. doi: 10.1093/genetics/107.2.179.
Abstract
We have identified mutations in seven unlinked genes (SPT genes) that affect the phenotypes of Ty and delta insertion mutations in the 5' noncoding region of the HIS4 gene of S. cerevisiae. Spt mutants were selected for suppression of his4-912 delta, a solo delta derivative of Ty912. Other Ty and delta insertions at HIS4 are suppressed by mutations in some but not all of the SPT genes. Only spt4 suppresses a non-Ty insertion at HIS4. In addition to their effects on Ty and delta insertions, mutations in several SPT genes show defects in general cellular functions--mating, DNA repair and growth.
摘要
我们已经在七个不连锁的基因（SPT基因）中鉴定出了突变，这些突变会影响酿酒酵母HIS4基因5'非编码区中Ty和δ插入突变的表型。选择Spt突变体来抑制his4-912δ，它是Ty912的一个单独的δ衍生物。HIS4处的其他Ty和δ插入在部分而非全部SPT基因中会被突变所抑制。只有spt4能抑制HIS4处的非Ty插入。除了对Ty和δ插入的影响外，几个SPT基因中的突变在一般细胞功能——交配、DNA修复和生长方面表现出缺陷。
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