Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
Ecotoxicol Environ Saf. 2012 Feb;76(2):126-34. doi: 10.1016/j.ecoenv.2011.10.007. Epub 2011 Oct 21.
The present study was conducted to determine interactive effects of waterborne co-exposure of copper (Cu) and calcium (Ca) on Cu accumulation, enzymatic activities and histology in yellow catfish Pelteobagrus fulvidraco and test the prediction that Ca could protect against Cu--induced toxicity in the fish species. Yellow catfish were exposed to 0, 1.0, 2.0 mg Cu/l, in combination with 0 and 50 mg Ca/l. Waterborne Cu and Ca co-exposure influenced the majority of tested enzymatic activities (succinate dehydrogenase, malic dehydrogenase, lactate dehydrogenase, lipoprotein lipase and hepatic lipase), and changed Cu contents in several organs (gill, liver, kidney, gastrointestine and muscle). For histological observations, at the same Ca level, waterborne Cu exposure induced injuries in gills and liver. However, Ca addition seemed to mitigate the severity of Cu--induced injuries. Thus, our study demonstrated that Ca had the capacity to reduce Cu toxicity in P. fulvidraco.
本研究旨在确定铜（Cu）和钙（Ca）在水中的共同暴露对黄颡鱼（Pelteobagrus fulvidraco）中 Cu 积累、酶活性和组织学的交互影响，并验证 Ca 可以保护鱼类免受 Cu 诱导的毒性这一预测。黄颡鱼暴露于 0、1.0、2.0 mg Cu/l，同时暴露于 0 和 50 mg Ca/l。水相 Cu 和 Ca 的共同暴露影响了大多数测试的酶活性（琥珀酸脱氢酶、苹果酸脱氢酶、乳酸脱氢酶、脂蛋白脂肪酶和肝脂肪酶），并改变了几个器官（鳃、肝、肾、胃肠道和肌肉）中的 Cu 含量。对于组织学观察，在相同的 Ca 水平下，水相 Cu 暴露会导致鳃和肝脏损伤。然而，添加 Ca 似乎减轻了 Cu 诱导损伤的严重程度。因此，我们的研究表明，Ca 有能力降低 P. fulvidraco 中的 Cu 毒性。
