He Xiaohong, Chen Xiaofei, Zhang Wenbin, Pu Yabin, Song Shen, Han Jianlin, Dong Kunzhe, Zhao Qianjun, Guan Weijun, Ma Yuehui, Jiang Lin
a The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China , Institute of Animal Science, Chinese Academy of Agricultural Science (CAAS) , Beijing , China.
b CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources , Institute of Animal Science, Chinese Academy of Agricultural Science (CAAS) , Beijing , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Nov;28(6):851-854. doi: 10.1080/24701394.2016.1197219. Epub 2016 Dec 10.
Heteroplasmy is the presence of more than one mitochondrial DNA (mtDNA) variant within a cell, tissue, or individual. In this study, sequence variation was investigated in the control region (CR) of mitochondrial DNA (mtDNA) from 135 individuals belonging to five primary domestic Bactrian camel breeds in China and Mongolia. Due to variation of the repeat unit G(T/C)(AC)n, length heteroplasmy was detected within each camel by direct sequencing and fragment analysis. A high occurrence of mtDNA heteroplasmy, up to 100 percentages was observed in five camel populations. Our study provides the first evidence of extensive length heteroplasmy in Bactrian camels.
异质性是指在一个细胞、组织或个体中存在不止一种线粒体DNA（mtDNA）变体。在本研究中，对来自中国和蒙古五个主要家养双峰驼品种的135个个体的线粒体DNA（mtDNA）控制区（CR）的序列变异进行了研究。由于重复单元G(T/C)(AC)n的变异，通过直接测序和片段分析在每头骆驼中检测到长度异质性。在五个骆驼群体中观察到mtDNA异质性的高发生率，高达100%。我们的研究首次提供了双峰驼中广泛存在长度异质性的证据。
