Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Hum Immunol. 2012 Jan;73(1):101-6. doi: 10.1016/j.humimm.2011.10.005. Epub 2011 Oct 12.
The aim of this study was to detect the association between 2 single nucleotide polymorphisms (SNPs), rs2910164 G>C and rs3746444 T>C, in pre-miRNA (hsa-mir-146a and hsa-mir-499) and the chronic inflammation in the Chinese Han population with rheumatoid arthritis (RA). Two hundred sixty-two Han Chinese patients with RA were recruited in this study. The SNPs were genotyped by polymerase chain reaction restriction fragment length polymorphism. C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and the plasma concentrations of interleukin (IL)-6, tumor necrosis factor α (TNF-α), and transforming growth factor β1 (TGF-β1) were measured. There was a significant difference in the levels of CRP and ESR among different genotypes in rs3746444 (p = 0.031 and p = 0.047, respectively). The heterozygote CT had significantly higher levels of CRP and ESR compared with homozygotes CC and TT. No significant association was observed between the SNP rs2910164 and the levels of CRP, ESR, IL-6, TNF-α, and TGF-β1 (all p > 0.05). The results of this study provided the first evidence that the SNP rs3746444 in pre-miR-499 could affect the inflammatory reaction in patients with RA. The findings were significant and might contribute to the clinical assessment of inflammatory activity, which in turn may influence therapeutic decision making.
本研究旨在检测汉族类风湿关节炎（RA）患者前体 miRNA（hsa-mir-146a 和 hsa-mir-499）中 2 个单核苷酸多态性（SNP）rs2910164 G>C 和 rs3746444 T>C 与慢性炎症之间的关联。本研究纳入了 262 例汉族 RA 患者。采用聚合酶链反应-限制性片段长度多态性方法检测 SNP。检测 C 反应蛋白（CRP）、红细胞沉降率（ESR）以及白细胞介素（IL）-6、肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）的血浆浓度。rs3746444 不同基因型之间 CRP 和 ESR 水平存在显著差异（p=0.031 和 p=0.047）。杂合子 CT 的 CRP 和 ESR 水平明显高于纯合子 CC 和 TT。SNP rs2910164 与 CRP、ESR、IL-6、TNF-α和 TGF-β1 水平之间无显著相关性（均 p＞0.05）。本研究结果首次提供证据表明，pre-miR-499 中的 SNP rs3746444 可能影响 RA 患者的炎症反应。这些发现具有重要意义，可能有助于炎症活动的临床评估，从而影响治疗决策。
