Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong 999077, China.
Genes (Basel). 2023 Feb 8;14(2):431. doi: 10.3390/genes14020431.
MicroRNAs (miRNAs) are small non-coding RNAs that play a key role in post-transcriptional modulation of individual genes' expression. Several miRNA variants from different populations are known to be associated with an increased risk of rheumatoid arthritis (RA).
This study was undertaken with the aim to investigate the association of single nucleotide variants; namely, rs2292832, rs3746444, rs11614913, rs1044165, and rs767649 of MIR149, MIR499, MIR196, MIR223, and MIR155, respectively, with RA in the Pakistani population.
A case-control study was performed by recruiting and genotyping a total of 600 individuals (300 cases and 300 controls) for these five variants using a TaqMan single-nucleotide polymorphism (SNP) genotyping assay. The resultant genotypic data was statistically analyzed through a chi-squared test for its association with RA under different inheritance models.
We found a significant association of rs2292832 with RA at genotypic (co-dominant ( < 0.0001), dominant (CC vs. TT + CT: OR 2.063 (1.437-2.962); = 0.0001), recessive (TT vs. CT + CC: OR 0.376 (0.259-0.548); < 0.0001)), and allelic (allele C) levels ((OR 0.506 (0.402-0637); < 0.0001)). Similarly, the rs3746444 showed a significant association with RA under co-dominant ( = 0.0001), dominant (GG vs. AA + AG: OR 5.246 (3.414-8.061); < 0.0001), recessive (AA vs. GG + AG: OR 0.653 (0.466-0.916); = 0.014), and additive models (G vs. A; OR 0.779 (0.620-0.978); = 0.03). However, we did not observe any significant association of rs11614913, rs1044165, or rs767649 with RA in our subjects.
To our knowledge, this was the first study that investigated and found an association between functional polymorphisms in miRNAs and RA in the Pakistani population.
microRNAs（miRNAs）是在个体基因表达的转录后调节中起关键作用的小型非编码 RNA。已知来自不同人群的几种 miRNA 变体与类风湿关节炎（RA）的风险增加有关。
本研究旨在探讨单核苷酸变异的相关性；即 rs2292832、rs3746444、rs11614913、rs1044165 和 rs767649，分别为 MIR149、MIR499、MIR196、MIR223 和 MIR155，与巴基斯坦人群中的 RA 相关。
通过 TaqMan 单核苷酸多态性（SNP）基因分型检测，对总共 600 名个体（300 名病例和 300 名对照）进行了这 5 个变体的病例对照研究。通过卡方检验对不同遗传模式下与 RA 相关的基因型数据进行了统计学分析。
我们发现 rs2292832 与 RA 存在显著相关性，表现在基因型（共显性（ < 0.0001）、显性（CC 与 TT+CT：OR 2.063（1.437-2.962）； = 0.0001）、隐性（TT 与 CT+CC：OR 0.376（0.259-0.548）； < 0.0001））和等位基因（等位基因 C）水平（（OR 0.506（0.402-0637）； < 0.0001））。同样，rs3746444 在共显性（ = 0.0001）、显性（GG 与 AA+AG：OR 5.246（3.414-8.061）； < 0.0001）、隐性（AA 与 GG+AG：OR 0.653（0.466-0.916）； = 0.014）和加性模型（G 与 A；OR 0.779（0.620-0.978）； = 0.03）中与 RA 存在显著相关性。然而，我们在研究对象中没有发现 rs11614913、rs1044165 或 rs767649 与 RA 之间的任何显著相关性。
据我们所知，这是第一项研究，在巴基斯坦人群中发现了 miRNA 中功能性多态性与 RA 之间的关联。
