蛋白质进入叶绿体的马达。
The motors of protein import into chloroplasts.
机构信息
Department of Plant Biology, University of California, Davis, CA, USA.
出版信息
Plant Signal Behav. 2011 Sep;6(9):1397-401. doi: 10.4161/psb.6.9.16916.
Abstract
Chloroplast function is largely dependent on its resident proteins, most of which are encoded by the nuclear genome and are synthesized in cytosol. Almost all of these are imported through the translocons located in the outer and inner chloroplast envelope membranes. The motor protein that provides the driving force for protein import has been proposed to be Hsp93, a member of the Hsp100 family of chaperones residing in the stroma. Combining in vivo and in vitro approaches, recent publications have provided multiple lines of evidence demonstrating that a stromal Hsp70 system is also involved in protein import into this organelle. Thus it appears that protein import into chloroplasts is driven by two motor proteins, Hsp93 and Hsp70. A perspective on collaboration between these two chaperones is discussed.
摘要
叶绿体的功能在很大程度上依赖于其固有的蛋白质，其中大部分由核基因组编码，并在细胞质中合成。这些蛋白质几乎都是通过位于叶绿体内外膜上的转运体进入叶绿体的。为蛋白质输入提供驱动力的运动蛋白被认为是 Hsp93，它是位于基质中的 Hsp100 家族伴侣蛋白的一员。结合体内和体外方法，最近的出版物提供了多条证据，证明基质中的 Hsp70 系统也参与了该细胞器的蛋白质输入。因此，似乎有两种运动蛋白 Hsp93 和 Hsp70 驱动蛋白质进入叶绿体。本文讨论了这两种伴侣蛋白之间的合作关系。
相似文献
1
The motors of protein import into chloroplasts.蛋白质进入叶绿体的马达。
Plant Signal Behav. 2011 Sep;6(9):1397-401. doi: 10.4161/psb.6.9.16916.
2
Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts.基质 HSP70 对于蛋白质易位进入豌豆和拟南芥叶绿体是重要的。
Plant Cell. 2010 May;22(5):1516-31. doi: 10.1105/tpc.109.071415. Epub 2010 May 18.
3
Molecular chaperone involvement in chloroplast protein import.分子伴侣参与叶绿体蛋白输入。
Biochim Biophys Acta. 2013 Feb;1833(2):332-40. doi: 10.1016/j.bbamcr.2012.03.019. Epub 2012 Apr 12.
4
Functional Analysis of the Hsp93/ClpC Chaperone at the Chloroplast Envelope.叶绿体被膜上Hsp93/ClpC伴侣蛋白的功能分析
Plant Physiol. 2016 Jan;170(1):147-62. doi: 10.1104/pp.15.01538. Epub 2015 Nov 19.
5
A stromal Hsp100 protein is required for normal chloroplast development and function in Arabidopsis.拟南芥中正常叶绿体发育和功能需要一种基质Hsp100蛋白。
Plant Physiol. 2004 Nov;136(3):3605-15. doi: 10.1104/pp.104.052928. Epub 2004 Oct 29.
6
The amino-terminal domain of chloroplast Hsp93 is important for its membrane association and functions in vivo.叶绿体 Hsp93 的氨基末端结构域对于其膜结合和体内功能很重要。
Plant Physiol. 2012 Apr;158(4):1656-65. doi: 10.1104/pp.112.193300. Epub 2012 Feb 21.
7
Precursors with altered affinity for Hsp70 in their transit peptides are efficiently imported into chloroplasts.在其转运肽中对Hsp70亲和力改变的前体被有效地导入叶绿体。
J Biol Chem. 2003 Nov 21;278(47):46473-81. doi: 10.1074/jbc.M306684200. Epub 2003 Sep 10.
8
An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts.叶绿体热休克蛋白 90（Hsp90C）在叶绿体蛋白质导入中的重要作用。
Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3173-8. doi: 10.1073/pnas.1219229110. Epub 2013 Feb 4.
9
Chloroplastic Hsp100 chaperones ClpC2 and ClpD interact in vitro with a transit peptide only when it is located at the N-terminus of a protein.类囊体热休克蛋白 100 伴侣 ClpC2 和 ClpD 在体外仅在多肽位于蛋白质 N 末端时相互作用。
BMC Plant Biol. 2012 Apr 30;12:57. doi: 10.1186/1471-2229-12-57.
10
Alternative processing of Arabidopsis Hsp70 precursors during protein import into chloroplasts.拟南芥Hsp70前体在蛋白质导入叶绿体过程中的可变加工
Biosci Biotechnol Biochem. 2008 Nov;72(11):2926-35. doi: 10.1271/bbb.80408. Epub 2008 Nov 7.
引用本文的文献
1
Chloroplast Functionality at the Interface of Growth, Defense, and Genetic Innovation: A Multi-Omics and Technological Perspective.生长、防御与基因创新界面的叶绿体功能：多组学与技术视角
Plants (Basel). 2025 Mar 20;14(6):978. doi: 10.3390/plants14060978.
2
Uncoupling the Threading and Unfoldase Actions of HSP101 Reveals Differences in Export between Soluble and Insoluble Proteins.解开 HSP101 的贯穿和展开酶活性揭示了可溶性蛋白和不可溶性蛋白在输出方面的差异。
mBio. 2019 Jun 4;10(3):e01106-19. doi: 10.1128/mBio.01106-19.
3
A Protochlorophyllide (Pchlide) Oxygenase for Plant Viability.一种对植物生存能力至关重要的原叶绿素酸酯（Pchlide）加氧酶。
Front Plant Sci. 2019 May 15;10:593. doi: 10.3389/fpls.2019.00593. eCollection 2019.
4
Multi-layered molecular mechanisms of polypeptide holding, unfolding and disaggregation by HSP70/HSP110 chaperones.热休克蛋白 70/110 伴侣通过多层次分子机制稳定、展开和去聚集多肽。
Front Mol Biosci. 2015 Jun 5;2:29. doi: 10.3389/fmolb.2015.00029. eCollection 2015.
5
Non-native, N-terminal Hsp70 molecular motor recognition elements in transit peptides support plastid protein translocation.转运肽中非天然的N端Hsp70分子运动识别元件支持质体蛋白转运。
J Biol Chem. 2015 Mar 20;290(12):7602-21. doi: 10.1074/jbc.M114.633586. Epub 2015 Feb 2.
6
New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development.叶绿体蛋白质输入机制及其与蛋白质质量控制、细胞器生物发生和发育整合的新见解。
J Mol Biol. 2015 Mar 13;427(5):1038-1060. doi: 10.1016/j.jmb.2014.08.016. Epub 2014 Aug 28.
7
Molecular chaperones are nanomachines that catalytically unfold misfolded and alternatively folded proteins.分子伴侣是一种纳米机器，能够催化展开错误折叠和其他折叠形式的蛋白质。
Cell Mol Life Sci. 2014 Sep;71(17):3311-25. doi: 10.1007/s00018-014-1627-y. Epub 2014 Apr 24.
8
Protein import into the photosynthetic organelles of and its implications for primary plastid endosymbiosis.蛋白质导入[具体生物名称未给出]的光合细胞器及其对原始质体内共生的影响。
Symbiosis. 2012 Dec;58(1-3):99-107. doi: 10.1007/s13199-012-0202-2. Epub 2012 Dec 13.
9
An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts.叶绿体热休克蛋白 90（Hsp90C）在叶绿体蛋白质导入中的重要作用。
Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3173-8. doi: 10.1073/pnas.1219229110. Epub 2013 Feb 4.
本文引用的文献
1
Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts.基质 HSP70 对于蛋白质易位进入豌豆和拟南芥叶绿体是重要的。
Plant Cell. 2010 May;22(5):1516-31. doi: 10.1105/tpc.109.071415. Epub 2010 May 18.
2
The TOC complex: preprotein gateway to the chloroplast.TOC复合体：叶绿体的前体蛋白通道
Biochim Biophys Acta. 2010 Jun;1803(6):715-23. doi: 10.1016/j.bbamcr.2010.03.004. Epub 2010 Mar 11.
3
Protein transport into chloroplasts.蛋白质向叶绿体的转运。
Annu Rev Plant Biol. 2010;61:157-80. doi: 10.1146/annurev-arplant-042809-112222.
4
Protein import into chloroplasts: the Tic complex and its regulation.蛋白质导入叶绿体：Tic复合体及其调控
Biochim Biophys Acta. 2010 Jun;1803(6):740-7. doi: 10.1016/j.bbamcr.2010.01.015. Epub 2010 Jan 25.
5
A stromal heat shock protein 70 system functions in protein import into chloroplasts in the moss Physcomitrella patens.基质热休克蛋白 70 系统在苔藓植物Physcomitrella patens 的叶绿体蛋白导入中发挥作用。
Plant Cell. 2010 Jan;22(1):205-20. doi: 10.1105/tpc.109.071464. Epub 2010 Jan 8.
6
Chloroplast biogenesis: diversity and regulation of the protein import apparatus.叶绿体生物发生：蛋白质输入装置的多样性与调控
Curr Opin Cell Biol. 2009 Aug;21(4):494-500. doi: 10.1016/j.ceb.2009.03.004. Epub 2009 May 4.
7
Targeting of nucleus-encoded proteins to chloroplasts in plants.植物中细胞核编码蛋白向叶绿体的靶向运输
New Phytol. 2008 Jul;179(2):257-285. doi: 10.1111/j.1469-8137.2008.02452.x.
8
Alternative processing of Arabidopsis Hsp70 precursors during protein import into chloroplasts.拟南芥Hsp70前体在蛋白质导入叶绿体过程中的可变加工
Biosci Biotechnol Biochem. 2008 Nov;72(11):2926-35. doi: 10.1271/bbb.80408. Epub 2008 Nov 7.
9
Protein trafficking to plastids: one theme, many variations.蛋白质向质体的运输：一个主题，多种变体。
Biochem J. 2008 Jul 1;413(1):15-28. doi: 10.1042/BJ20080490.
10
Functional similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein (Hip).叶绿体转运体组分Tic40与人类共伴侣蛋白Hsp70相互作用蛋白（Hip）之间的功能相似性。
J Biol Chem. 2007 Jul 20;282(29):21404-14. doi: 10.1074/jbc.M611545200. Epub 2007 May 29.
Zotero 插件