Center of Human Genetics, University of Bremen, Leobener Strasse ZHG, D-28359 Bremen, Germany; Small Animal Clinic, University of Veterinary Medicine and Research Cluster of Excellence "REBIRTH", Bünteweg 9, D-30559 Hannover, Germany.
Cytokine. 2011 Dec;56(3):811-6. doi: 10.1016/j.cyto.2011.09.025. Epub 2011 Oct 22.
BMP4 has been linked to early steps of adipocyte lineage differentiation but only little is known about its corresponding downstream pathways. Herein, we have investigated whether or not the expression of high mobility group protein HMGA2, another protein linked to proliferation and differentiation within the process of adipogenesis, may be influenced by BMP4 signaling in adipose tissue derived stem cells. Compared to FGF1, a strong inducer of HMGA2 in immortalized pre-adipocytes, BMP4 was found moderately to induce the HMGA2 mRNA expression in serum starved adipose tissue derived stem cells and myometrial cells. In contrast, no such activity was noted in canine bone marrow derived mesenchymal stem cells. As to adipocyte lineage differentiation the functions of BMP4 and HMGA2 mechanistically overlap. Thus, we propose that in adipose tissue BMP4 acts in part by activating HMGA2 making this architectural transcription factor one of the major downstream players in that system.
BMP4 与脂肪细胞谱系分化的早期步骤有关，但人们对其相应的下游途径知之甚少。在此，我们研究了在脂肪组织来源的干细胞中，BMP4 信号是否会影响与增殖和脂肪生成过程中的分化有关的另一种蛋白质高迁移率族蛋白 HMGA2 的表达。与强烈诱导永生化前脂肪细胞中 HMGA2 表达的 FGF1 相比，BMP4 被发现适度诱导血清饥饿的脂肪组织来源的干细胞和子宫细胞中 HMGA2 mRNA 的表达。相比之下，在犬骨髓间充质干细胞中未观察到这种活性。就脂肪细胞谱系分化而言，BMP4 和 HMGA2 的功能在机制上重叠。因此，我们提出，在脂肪组织中，BMP4 通过激活 HMGA2 发挥作用，使这种结构转录因子成为该系统中的主要下游参与者之一。
