Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Am J Phys Med Rehabil. 2011 Dec;90(12):1020-9. doi: 10.1097/PHM.0b013e318238a166.
The aim of this study was to test the effects of physiologic ischemic training on the performance of skeletal muscle in a pathologically ischemic limb models.
Twenty-seven New Zealand white rabbits were randomly assigned to three groups: (1) those in the ischemia with cuff inflation training group received right femoral artery ligation and occlusion of blood flow in the left limb by 3 mins of cuff inflation followed by 5 mins of deflation, repeated three times; (2) those in the ischemia with isometric exercise training group received right femoral artery ligation and 4 mins of isometric contraction exercise ischemic training in the left limb followed by 10 mins of rest, repeated twice; (3) those in the sole ischemia group received right femoral artery ligation and no intervention in the left limb. Five weeks later, muscle endurance, resting blood flow, and collateral angiogenesis were examined. Capillary supply, types and cross-sectional areas of muscle fibers and cellular oxidative potential were also evaluated.
Gastronomic endurance of rabbits in the cuff inflation training group was the best among the three groups (P < 0.01). Collateral circulation (P < 0.05), distribution of type I fibers (P < 0.01), and cross-sectional area of type IIB fibers (P < 0.05) were also significantly higher in the cuff inflation training group than in the sole ischemia group. Similar differences were recorded between the isometric exercise training and sole ischemia groups. The distribution of type IIA fibers was lower (P < 0.01) in the cuff inflation training group than in the isometric exercise training group. Distribution of type IIB was found to be significantly different between these two groups (P < 0.01).
Physiologic ischemic exercise training can promote remote angiogenesis in the pathologic ischemic skeletal muscle and thus improve performance.
本研究旨在测试生理缺血训练对病理性缺血肢体模型中骨骼肌性能的影响。
27 只新西兰白兔随机分为三组：（1）缺血加袖带充气训练组接受右股动脉结扎和左肢血流阻断 3 分钟，随后袖带放气 5 分钟，重复 3 次；（2）缺血等长运动训练组接受右股动脉结扎和左肢 4 分钟等长收缩缺血训练，随后休息 10 分钟，重复 2 次；（3）单纯缺血组接受右股动脉结扎和左肢无干预。5 周后，检测肌肉耐力、静息血流和侧支血管生成。还评估了毛细血管供应、肌纤维类型和横截面积以及细胞氧化潜能。
袖带充气训练组的兔子胃耐力在三组中最好（P<0.01）。侧支循环（P<0.05）、I 型纤维分布（P<0.01）和 IIB 型纤维横截面积（P<0.05）在袖带充气训练组也明显高于单纯缺血组。等长运动训练组与单纯缺血组之间也记录到类似的差异。袖带充气训练组 IIA 型纤维分布较低（P<0.01）。两组间 IIB 型纤维分布存在显著差异（P<0.01）。
生理性缺血运动训练可促进病理性缺血骨骼肌的远程血管生成，从而提高性能。
